Team:Groningen/Labwork/10 September 2013

From 2013.igem.org

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<br>ColonyPCR on 5 colonies per plate was performed using VF2 and VR primers (annealing temperature 58&deg;C).
<br>ColonyPCR on 5 colonies per plate was performed using VF2 and VR primers (annealing temperature 58&deg;C).
<br>The samples were checked on agarose gel 0.8% beside the positive control (pSB1C3-S1-S5).
<br>The samples were checked on agarose gel 0.8% beside the positive control (pSB1C3-S1-S5).
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<h2>Sebas</h2>
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All plates (containing 5µg/ml cm) show a smear of 'death' cells.
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One single colonies were only visible on the non-control plates.
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For GFP0840 there were ~20 colonies for RFP 1 colonie and for DSMgfp no colonie.
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Restreaked colonies on LB/Starch/CM plates.
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<Br>
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<br>
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<Br>Did digestion check on GFP0840 1&3, GFPdsm 1&3, RFP 1&4 with XhoI (digest in promoter and outside)
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<br>When promoter would not be there XhoI would cut 1 time, when promoter is there it would could twice.
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<p>
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<img src="https://static.igem.org/mediawiki/2013/a/af/840-1-3%3B_dsm-1-3%3B_rfp-1-4_seperated_more.jpg" width="60%"></img>
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Revision as of 11:37, 10 September 2013

Mirjam

Genomic DNA extraction for ΔCheY and ΔDes.

Claudio

pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 plates showed colonies.
ColonyPCR on 5 colonies per plate was performed using VF2 and VR primers (annealing temperature 58°C).
The samples were checked on agarose gel 0.8% beside the positive control (pSB1C3-S1-S5).

Sebas

All plates (containing 5µg/ml cm) show a smear of 'death' cells. One single colonies were only visible on the non-control plates. For GFP0840 there were ~20 colonies for RFP 1 colonie and for DSMgfp no colonie. Restreaked colonies on LB/Starch/CM plates.


Did digestion check on GFP0840 1&3, GFPdsm 1&3, RFP 1&4 with XhoI (digest in promoter and outside)
When promoter would not be there XhoI would cut 1 time, when promoter is there it would could twice.