Team:Wageningen UR/Backbone enzyme database

From 2013.igem.org

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<p>In general, the biosynthesis genes for fungal secondary metabolites are located in clusters. The fact that secondary metabolites are often synthesized as polymer backbones that are subsequently diversified greatly via the actions of tailoring enzymes sets the stage for combinatorial biochemistry because their biosynthesis is modular. In this combinatorial approach in which a modular system of domain shuffling can be used to generate a plethora of novel enzyme variants with new and improved functionalities. The number of permutations is extensive as there is a myriad of different domains that can be added, removed, reordered or exchanged. In order to facilitate mixing and matching of domains of these large enzymes a database for Aspergilli will be created. </p>
<p>In general, the biosynthesis genes for fungal secondary metabolites are located in clusters. The fact that secondary metabolites are often synthesized as polymer backbones that are subsequently diversified greatly via the actions of tailoring enzymes sets the stage for combinatorial biochemistry because their biosynthesis is modular. In this combinatorial approach in which a modular system of domain shuffling can be used to generate a plethora of novel enzyme variants with new and improved functionalities. The number of permutations is extensive as there is a myriad of different domains that can be added, removed, reordered or exchanged. In order to facilitate mixing and matching of domains of these large enzymes a database for Aspergilli will be created. </p>
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Revision as of 09:31, 10 September 2013

Backbone enzyme database

Enzyme database

on secondary metabolites backbone enzymes from Aspergilli



Introduction

In general, the biosynthesis genes for fungal secondary metabolites are located in clusters. The fact that secondary metabolites are often synthesized as polymer backbones that are subsequently diversified greatly via the actions of tailoring enzymes sets the stage for combinatorial biochemistry because their biosynthesis is modular. In this combinatorial approach in which a modular system of domain shuffling can be used to generate a plethora of novel enzyme variants with new and improved functionalities. The number of permutations is extensive as there is a myriad of different domains that can be added, removed, reordered or exchanged. In order to facilitate mixing and matching of domains of these large enzymes a database for Aspergilli will be created.

Rationale

Aim

Approach

Research Methods

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