"
Team:DTU-Denmark/Notebook/10 September 2013
From 2013.igem.org
(Difference between revisions)
(→Gel) |
(→Gel) |
||
| Line 24: | Line 24: | ||
* 1 kb ladder | * 1 kb ladder | ||
| - | * colony 1, primer pair 1 | + | * Nir colony 1, primer pair 1 |
| - | * colony 1, primer pair 2 | + | * Nir colony 1, primer pair 2 |
| - | * colony 2, primer pair 1 | + | * Nir colony 2, primer pair 1 |
| - | * colony 2, primer pair 2 | + | * Nir colony 2, primer pair 2 |
| - | * colony 3, primer pair 1 | + | * Nir colony 3, primer pair 1 |
| - | * colony 3, primer pair 2 | + | * Nir colony 3, primer pair 2 |
| - | * colony 4, primer pair 1 | + | * Nir colony 4, primer pair 1 |
| - | * colony 5, primer pair 2 | + | * Nir colony 5, primer pair 2 |
| - | * colony 5, primer pair 1 | + | * Nir colony 5, primer pair 1 |
| - | * colony 5, primer pair 2 | + | * Nir colony 5, primer pair 2 |
| - | * colony 6, primer pair 1 | + | * Nir colony 6, primer pair 1 |
| - | * colony 6, primer pair 2 | + | * Nir colony 6, primer pair 2 |
* 1 kb ladder | * 1 kb ladder | ||
Revision as of 12:12, 11 September 2013
10 September
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR to verify Nir in pZA21::ara-tight
Redid colony PCR with sequencing primers instead. Two primer pairs to test for the two USER parts. Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.
Used Q5 premix.
Results
Gel
- 1 kb ladder
- Nir colony 1, primer pair 1
- Nir colony 1, primer pair 2
- Nir colony 2, primer pair 1
- Nir colony 2, primer pair 2
- Nir colony 3, primer pair 1
- Nir colony 3, primer pair 2
- Nir colony 4, primer pair 1
- Nir colony 5, primer pair 2
- Nir colony 5, primer pair 1
- Nir colony 5, primer pair 2
- Nir colony 6, primer pair 1
- Nir colony 6, primer pair 2
- 1 kb ladder
