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Team:DTU-Denmark/Notebook/10 September 2013
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Used Q5 premix. Calculated annealing temperature for pair 1: 60C, pair 2: 64C | Used Q5 premix. Calculated annealing temperature for pair 1: 60C, pair 2: 64C | ||
| - | Note: The resulting gel showed a lot of bands that are consistent for all colonies. We repeated the PCR for one of the colonies with the following annealing temperatures: pair 1: 67C, pair 2: 68C | + | Note: The resulting gel showed a lot of bands that are consistent for all colonies. We repeated the PCR on the next day for one of the colonies with the following annealing temperatures: pair 1: 67C, pair 2: 68C |
==Results== | ==Results== | ||
Revision as of 14:44, 11 September 2013
10 September
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR to verify Nir in pZA21::ara-tight
Redid colony PCR with sequencing primers instead. Two primer pairs to test for the two USER parts. Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.
Used Q5 premix. Calculated annealing temperature for pair 1: 60C, pair 2: 64C
Note: The resulting gel showed a lot of bands that are consistent for all colonies. We repeated the PCR on the next day for one of the colonies with the following annealing temperatures: pair 1: 67C, pair 2: 68C
Results
Gel
- 1 kb ladder
- Nir colony 1, primer pair 1
- Nir colony 1, primer pair 2
- Nir colony 2, primer pair 1
- Nir colony 2, primer pair 2
- Nir colony 3, primer pair 1
- Nir colony 3, primer pair 2
- Nir colony 4, primer pair 1
- Nir colony 5, primer pair 2
- Nir colony 5, primer pair 1
- Nir colony 5, primer pair 2
- Nir colony 6, primer pair 1
- Nir colony 6, primer pair 2
- 1 kb ladder
