Team:Groningen/Labwork/12 September 2013

From 2013.igem.org

(Difference between revisions)
Line 27: Line 27:
<br>Colony PCR was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 58&deg;C).
<br>Colony PCR was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 58&deg;C).
<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
 +
 +
<h2>Mirjam</h2>
 +
Did a ligation of the silk plus signal sequences (made by Sander) to the registration backbone.
 +
<br>Saw colonies on the plates of complete transformation of the &Delta;CheY mutant to our <i>B. subtilis</i> 168 strain.
 +
</div>
</div>

Revision as of 11:39, 12 September 2013

Sebas

Claudio

The plates pSB1C3-S1-S5-S5 and pSB1C3-S2-S5-S5 showed thousands of colonies.
Colony PCR was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 58°C).
The samples were checked on agarose gel 0.8%.

Mirjam

Did a ligation of the silk plus signal sequences (made by Sander) to the registration backbone.
Saw colonies on the plates of complete transformation of the ΔCheY mutant to our B. subtilis 168 strain.