Team:XMU-China/Content

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By constructing robust circuits in E.coli, we want to build a gene network capable of synchronizing genetic oscillations in multiple levels. Cells can be synchronized at the colony level via quorum sensing, and a gas-phase redox will be signaling (mainly H2O2) between colonies simultaneously. Two scales of coupling ensured extremely consistent oscillations.
By constructing robust circuits in E.coli, we want to build a gene network capable of synchronizing genetic oscillations in multiple levels. Cells can be synchronized at the colony level via quorum sensing, and a gas-phase redox will be signaling (mainly H2O2) between colonies simultaneously. Two scales of coupling ensured extremely consistent oscillations.
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So far the circuit construction has already been accomplished, and their performances are under testing right now. On a liquid crystal display (LCD)-like microfluidic array bacteria grow in separate colonies, so that synchronization in single colony and gas-phase communication among different colonies could be verified.
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<p>Reactive oxygen species (ROS) are considered the main factor in synchronizing separate colonies, so organisms that can generate ROS will have an impact on our oscillation. By measuring different fluorescent signals (GFP is used as reporter), quantitative analysis of ROS in inflammation or malignant tissues is our ultimate aim. Quorum sensing can lead communication over tens of micrometers. However, coordination between colonies over millimeter scales is better at environmental sensitivity owing to its larger cell number, which made our devices a hypersensitive detector in heavy metals and pathogens.
<p>Reactive oxygen species (ROS) are considered the main factor in synchronizing separate colonies, so organisms that can generate ROS will have an impact on our oscillation. By measuring different fluorescent signals (GFP is used as reporter), quantitative analysis of ROS in inflammation or malignant tissues is our ultimate aim. Quorum sensing can lead communication over tens of micrometers. However, coordination between colonies over millimeter scales is better at environmental sensitivity owing to its larger cell number, which made our devices a hypersensitive detector in heavy metals and pathogens.
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Revision as of 12:21, 14 September 2013

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XMU-iGEM 2013

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By constructing robust circuits in E.coli, we want to build a gene network capable of synchronizing genetic oscillations in multiple levels. Cells can be synchronized at the colony level via quorum sensing, and a gas-phase redox will be signaling (mainly H2O2) between colonies simultaneously. Two scales of coupling ensured extremely consistent oscillations.

Reactive oxygen species (ROS) are considered the main factor in synchronizing separate colonies, so organisms that can generate ROS will have an impact on our oscillation. By measuring different fluorescent signals (GFP is used as reporter), quantitative analysis of ROS in inflammation or malignant tissues is our ultimate aim. Quorum sensing can lead communication over tens of micrometers. However, coordination between colonies over millimeter scales is better at environmental sensitivity owing to its larger cell number, which made our devices a hypersensitive detector in heavy metals and pathogens.

Proin tincidunt ipsum sed quam convallis rhoncus.

Suspendisse at libero eu magna pharetra tincidunt vel vel dui. Nunc leo nibh, suscipit vel venenatis vel, fermentum at cursus sed, suscipit id massa dapibus vitae neque.

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