Team:Groningen/Labwork/13 September 2013

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<br>Colony C from pSB1C3-S16-3-5 and colony D from pSB1C3-S16-9-11 were positive candidate and were inoculated in LB + Cm.
<br>Colony C from pSB1C3-S16-3-5 and colony D from pSB1C3-S16-9-11 were positive candidate and were inoculated in LB + Cm.
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<br>Plates containing several combination of signal sequences together with S3 or S9 showed colonies.
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<br>Colony PCR was performed (NEB Mater Mix) on 2 colonies per plate using VF2 and VR primers (annealing temperature 64&deg;C).
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<h2>Inne</h2>
<h2>Inne</h2>

Revision as of 13:01, 14 September 2013

Claudio

The plates pSB1C3-S16-3-5 and pSB1C3-S16-9-11 showed around thousands of colonies.
Colony PCR was performed (NEB Mater Mix) on 4 colonies per plate using VF2 and VR primers (annealing temperature 64°C).

Colony C from pSB1C3-S16-3-5 and colony D from pSB1C3-S16-9-11 were positive candidate and were inoculated in LB + Cm.

Plates containing several combination of signal sequences together with S3 or S9 showed colonies.
Colony PCR was performed (NEB Mater Mix) on 2 colonies per plate using VF2 and VR primers (annealing temperature 64°C).

Inne

Ran 2 gels with colony pcr samples.
Gel 1:
Ladder s16-3-5 A s16-3-5 B s16-3-5 C s16-3-5 D s16-9-11 A s16-9-11 B s16-9-11 C s16-9-11 D Positive control


Gel 2
Ladder 1 A 1 B empty empty 2 A 2 B 3 A 3 B 4 A 4 B

































Ladder 5 A 5 B 6 A 6 B 7 A 7 B 8 A 8 B empty empty