Team:Groningen/Labwork/14 September 2013

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<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
<br><img src="https://static.igem.org/mediawiki/2013/0/09/ColonyPCR_BBa_K1085014-silk.jpg" width="50%">
<br><img src="https://static.igem.org/mediawiki/2013/0/09/ColonyPCR_BBa_K1085014-silk.jpg" width="50%">
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<br>Colony B from plate BBa_K1085014-S2-S5-S13 showed a band at the expected height (). Therefore was inoculated in LB + Cm.
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<br>Colony B from plate BBa_K1085014-S2-S5-S13 showed a band at the expected height (~2400 bps). Therefore was inoculated in LB + Cm.
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<br>Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed thousands of colonies.
<br>Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed thousands of colonies.

Revision as of 08:31, 15 September 2013

Claudio

Plates containing BBa_K1085014-S1-S5-S14 and BBa_K1085014-S2-S5-S13 showed around 20 colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using F_spank_NheI and HM14 primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Colony B from plate BBa_K1085014-S2-S5-S13 showed a band at the expected height (~2400 bps). Therefore was inoculated in LB + Cm.

Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Colony C from pSB1C3-S1-S5-S13 and colony A from pSB1C3-S2-S5-S14 show to be positive candidates and were inoculated in LB + Cm.

S13 and S14 were ligated into pSB1C3-S1-S5-S5 and pSB1C3-S2-S5-S5, respectively.
S13 and S14 were ligated into pSB1C3-S16-S3-S5 and pSB1C3-S16-S9-S11, respectively.
The ligation products were plated on LB + Cm.