Template:Team:Uppsala/JS/notebook

  ds = '<div id="dairy-text"><br><h1>Wednesday 2013-07-10</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Karl H, Peter C., Lovisa P, Ken B-A, Alexander, Niclas, Magnus, Nils, Thorsteinn, Christoffer Ahlström (CA),  Anton Berglund (AB), Stephanie Herman (SH), Viktor Törnblom (VT)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>96. p?A?-Cs42s<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB3K3-CP44-B0032-BFP<br>87. pSB3K3-CP1-B0032-BFP<br>88. pSB3K3-CP41-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><h2>Todays work</h2><br><b>Digestion:<b> <br>22. pSB1C3-RBS-B0032-BFP<br><br><b>Ligation:</b><br>44. pSB1C3-B0034-His-TAL<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB3K3-CP44-B0032-BFP<br>87. pSB3K3-CP1-B0032-BFP<br>88. pSB3K3-CP41-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>transformation:</b><br>44. pSB1C3-B0034-His-TAL<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB3K3-CP44-B0032-BFP<br>87. pSB3K3-CP1-B0032-BFP<br>88. pSB3K3-CP41-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Re-streak</b> <br>96. p?A?-cs42s<br><i>-Streak of transformations of ligated 20 and positive controll (AV,NA)<br>PCR of 19(digested EP), 20(digested EP), 21(digested EP) and CrtO(from 21digestEP) (AV,NA)</i><br><br><b>Spreading of strains: </b><br>Lb4. reuteri 100-23 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br>Lb10. plantarum 256 p256<br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 noplasmid: Growth<br>Lb11. plantarum 36E noplasmid: Growth<br>→ Spread out (1x) on erythromycin plates, to examine whether they are naturally resistant.<br><br><h2>Results</h2><br><b>Transformation: </b> <br>Lb4. reuteri 100-23 noplasmid: Growth <br>Lb12. reuteri DSM 20016 noplasmid: Growth<br><br><b>Re-streak:</b> <br>96. p?A?-cs42s worked well. <br><br><b>O/N culture:</b><br>69.1 pSB3K3-CP8-B0032-BFP <br>69.2 pSB3K3-CP8-B0032-BFP <br>69.3 pSB3K3-CP8-B0032-BFP <br>69.4 pSB3K3-CP8-B0032-BFP <br>70.1 pSB3K3-CP11-B0032-BFP <br>70.2 pSB3K3-CP11-B0032-BFP <br>70.3 pSB3K3-CP11-B0032-BFP <br>70.4 pSB3K3-CP11-B0032-BFP <br>71.1 pSB3K3-CP29-B0032-BFP<br>71.2 pSB3K3-CP29-B0032-BFP<br>71.3 pSB3K3-CP29-B0032-BFP<br>71.4 pSB3K3-CP29-B0032-BFP<br>72.1 pSB3K3-CP30-B0032-BFP<br>72.2 pSB3K3-CP30-B0032-BFP<br>72.3 pSB3K3-CP30-B0032-BFP<br>72.4 pSB3K3-CP30-B0032-BFP<br>*Growth<br><br><b>Plasmid preparation: </b><br>69.1 pSB3K3-CP8-B0032-BFP: 80.5 ng/µl<br>69.2 pSB3K3-CP8-B0032-BFP: 75.7 ng/µl<br>69.3 pSB3K3-CP8-B0032-BFP: 60.5 ng/µl<br>69.4 pSB3K3-CP8-B0032-BFP: 50.8 ng/µl<br>70.1 pSB3K3-CP11-B0032-BFP: 71.7 ng/µl<br>70.2 pSB3K3-CP11-B0032-BFP: 56.3 ng/µl<br>70.3 pSB3K3-CP11-B0032-BFP: 56.0 ng/µl<br>70.4 pSB3K3-CP11-B0032-BFP: 45.3 ng/µl<br>71.1 pSB3K3-CP29-B0032-BFP: 54.0 ng/µl<br>71.2 pSB3K3-CP29-B0032-BFP: 49.1 ng/µl<br>71.3 pSB3K3-CP29-B0032-BFP: 83.0 ng/µl<br>71.4 pSB3K3-CP29-B0032-BFP 67.8 ng/µl<br>72.1 pSB3K3-CP30-B0032-BFP 53.8 ng/µl<br>72.2 pSB3K3-CP30-B0032-BFP 71.8 ng/µl<br>72.3 pSB3K3-CP30-B0032-BFP 109.6 ng/µl<br>72.4 pSB3K3-CP30-B0032-BFP 106.8 ng/µl<br></div>';