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Team:Grenoble-EMSE-LSU/Documentation/Notebook/June
From 2013.igem.org
(Difference between revisions)
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</p> | </p> | ||
<h3>Thursday</h3> | <h3>Thursday</h3> | ||
| + | <p>Failure with the transfections.</br> | ||
| + | Other problem with the petri dishes : everything grew, even the WT colonies that should have died because of antibiotics. Maybe the antibiotics are too old since they were used by Grenoble iGEM Team of 2012</p> | ||
<h3>Friday</h3> | <h3>Friday</h3> | ||
| - | < | + | <p> |
| + | Preparation of freezable competent cells (WT)(40 alicos of 100µL)</br> | ||
| + | Same transfections than wednesday, with fresh antibiotics.</br> | ||
| + | </br> | ||
| + | PCR on pAraBad, mCherry and KillerRed, only pAraBAD worked.</br></br> | ||
| + | <strong>Team meeting</strong></br> | ||
| + | TODO List :</br> | ||
| + | - Find a name</br> | ||
| + | - Begin construction of :</br> | ||
| + | ->pLac-RBS-YF1-fixJ</br> | ||
| + | ->pFixK2-RBS-GFP</br> | ||
| + | - glycerol-stock BBs</br> | ||
| + | - E-glometer, caracterisation of LED</br> | ||
| + | </p> | ||
<h2>Week 2</h2> | <h2>Week 2</h2> | ||
<p> | <p> | ||
Revision as of 18:03, 18 September 2013
