Template:Kyoto/Notebook/Aug 27

From 2013.igem.org

(Difference between revisions)
(Restriction Enzyme Digestion)
(Restriction Enzyme Digestion)
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<span class="author">No name</span>
<span class="author">No name</span>
{| class="wikitable"
{| class="wikitable"
-
!8/21 pT181 attenuator-1(330&micro;g/mL)||EcoRI||SpeI||10xBufferB||100xBSA||MilliQ||total
+
!8/21 pT181 attenuator-1(330&micro;g/mL)||EcoRI||SpeI||10x BufferB||100x BSA||MilliQ||total
|-
|-
|3.0&micro;L||0.5&micro;L||0.5&micro;L||3.0&micro;L||0.3&micro;L||22.7&micro;L||30&micro;L
|3.0&micro;L||0.5&micro;L||0.5&micro;L||3.0&micro;L||0.3&micro;L||22.7&micro;L||30&micro;L
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|}
|}
{| class="wikitable"
{| class="wikitable"
-
!8/17 DT-1(188&micro;g/mL)||EcoRI||XbaI||10xBufferB||100xBSA||MilliQ||total
+
!8/17 DT-1(188&micro;g/mL)||EcoRI||XbaI||10x BufferB||100x BSA||MilliQ||total
|-
|-
|3.0&micro;L||0.5&micro;L||0.5&micro;L||3.0&micro;L||0.3&micro;L||22.7&micro;L||30&micro;L
|3.0&micro;L||0.5&micro;L||0.5&micro;L||3.0&micro;L||0.3&micro;L||22.7&micro;L||30&micro;L
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|}
|}
{| class="wikitable"
{| class="wikitable"
-
!8/20 Pcon-RBS-luxR-DT-2(344&micro;g/mL)||EcoRI||SpeI||10xBufferB||100xBSA||MilliQ||total
+
!8/20 Pcon-RBS-luxR-DT-2(344&micro;g/mL)||EcoRI||SpeI||10x BufferB||100x BSA||MilliQ||total
|-
|-
|2.9&micro;L||0.5&micro;L||0.5&micro;L||3.0&micro;L||0.3&micro;L||22.8&micro;L||30&micro;L
|2.9&micro;L||0.5&micro;L||0.5&micro;L||3.0&micro;L||0.3&micro;L||22.8&micro;L||30&micro;L

Revision as of 03:17, 19 September 2013

Aug 27

Restriction Enzyme Digestion

No name

8/21 pT181 attenuator-1(330µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
0.6µL0µL0µL1µL0.1µL8.3µL10µL
8/17 DT-1(188µg/mL)EcoRIXbaI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
1.0µl0µL0µL1µL0.1µL7.9µL10µL
8/20 Pcon-RBS-luxR-DT-2(344µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
2.9µL0.5µL0.5µL3.0µL0.3µL22.8µL30µL
0.6µl0µL0µL1µL0.1µL8.3µL10µL