Template:Kyoto/Notebook/Aug 27
From 2013.igem.org
(Difference between revisions)
(→Restriction Enzyme Digestion) |
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<span class="author">No name</span> | <span class="author">No name</span> | ||
{| class="wikitable" | {| class="wikitable" | ||
- | !8/21 pT181 attenuator-1(330µg/mL)||EcoRI||SpeI|| | + | !8/21 pT181 attenuator-1(330µg/mL)||EcoRI||SpeI||10x BufferB||100x BSA||MilliQ||total |
|- | |- | ||
|3.0µL||0.5µL||0.5µL||3.0µL||0.3µL||22.7µL||30µL | |3.0µL||0.5µL||0.5µL||3.0µL||0.3µL||22.7µL||30µL | ||
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|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
- | !8/17 DT-1(188µg/mL)||EcoRI||XbaI|| | + | !8/17 DT-1(188µg/mL)||EcoRI||XbaI||10x BufferB||100x BSA||MilliQ||total |
|- | |- | ||
|3.0µL||0.5µL||0.5µL||3.0µL||0.3µL||22.7µL||30µL | |3.0µL||0.5µL||0.5µL||3.0µL||0.3µL||22.7µL||30µL | ||
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|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
- | !8/20 Pcon-RBS-luxR-DT-2(344µg/mL)||EcoRI||SpeI|| | + | !8/20 Pcon-RBS-luxR-DT-2(344µg/mL)||EcoRI||SpeI||10x BufferB||100x BSA||MilliQ||total |
|- | |- | ||
|2.9µL||0.5µL||0.5µL||3.0µL||0.3µL||22.8µL||30µL | |2.9µL||0.5µL||0.5µL||3.0µL||0.3µL||22.8µL||30µL |
Revision as of 03:17, 19 September 2013
Aug 27
Restriction Enzyme Digestion
8/21 pT181 attenuator-1(330µg/mL) | EcoRI | SpeI | 10x BufferB | 100x BSA | MilliQ | total |
---|---|---|---|---|---|---|
3.0µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.7µL | 30µL |
0.6µL | 0µL | 0µL | 1µL | 0.1µL | 8.3µL | 10µL |
8/17 DT-1(188µg/mL) | EcoRI | XbaI | 10x BufferB | 100x BSA | MilliQ | total |
---|---|---|---|---|---|---|
3.0µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.7µL | 30µL |
1.0µl | 0µL | 0µL | 1µL | 0.1µL | 7.9µL | 10µL |
8/20 Pcon-RBS-luxR-DT-2(344µg/mL) | EcoRI | SpeI | 10x BufferB | 100x BSA | MilliQ | total |
---|---|---|---|---|---|---|
2.9µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.8µL | 30µL |
0.6µl | 0µL | 0µL | 1µL | 0.1µL | 8.3µL | 10µL |