Template:Kyoto/Notebook/Aug 27

From 2013.igem.org

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===Electrophoresis===
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<div class="experiment">
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<span class="author">No name</span>
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{| class="wikitable"
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!Lane||Sample||Enzyme1||Enzyme2
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|-
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|1||100bp ladder||--||--
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|-
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|2||8/21 pT181 attenuator||EcoI||SpeI
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|-
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|3||8/21 pT181 atteniator||||--
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|-
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|4||8/17 DT-1||EcoRI||XbaI
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|-
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|5||8/17 DT-1||--||--
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|-
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|6||8/20 Pcon-RBS-luxR-DT-2||EcoI||SpeI
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|-
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|7||8/20 Pcon-RBS-luxR-DT-2||--||--
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|-
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|8||100bp ladder||--||--
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|}
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Revision as of 04:10, 19 September 2013

Aug 27

Restriction Enzyme Digestion

No name

8/21 pT181 attenuator-1(330µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
0.6µL0µL0µL1µL0.1µL8.3µL10µL
8/17 DT-1(188µg/mL)EcoRIXbaI10x BufferB100x BSAMilliQtotal
3.0µL0.5µL0.5µL3.0µL0.3µL22.7µL30µL
1.0µl0µL0µL1µL0.1µL7.9µL10µL
8/20 Pcon-RBS-luxR-DT-2(344µg/mL)EcoRISpeI10x BufferB100x BSAMilliQtotal
2.9µL0.5µL0.5µL3.0µL0.3µL22.8µL30µL
0.6µl0µL0µL1µL0.1µL8.3µL10µL

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp ladder----
28/21 pT181 attenuatorEcoISpeI
38/21 pT181 atteniator--
48/17 DT-1EcoRIXbaI
58/17 DT-1----
68/20 Pcon-RBS-luxR-DT-2EcoISpeI
78/20 Pcon-RBS-luxR-DT-2----
8100bp ladder----
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