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Team:Hong Kong HKUST/notebook/mod1
From 2013.igem.org
(Difference between revisions)
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| - | .content { display:none; background:#8FF5D5;border-radius: | + | .content { display:none; background:#8FF5D5;border-radius:100px;margin-bottom:5px;align:center;width:90%;z-index:-3;padding-left:100px;} |
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<div> | <div> | ||
<a href='#' class='head'>Week 4</a> | <a href='#' class='head'>Week 4</a> | ||
| - | <div class='content' align="left"> | + | <div class='content' align="left"> |
| - | + | <p> </p> | |
| - | + | <p><strong>June 25:</strong></p> | |
| - | + | <ul> | |
| - | + | <li>Prepared DMEM in TC room</li> | |
| - | + | <li>Added FBS (Serum) & Antibiotics into DMEM</li> | |
| - | + | </ul> | |
| + | <p> </p> | ||
| + | <p><strong>June 27:</strong></p> | ||
| + | <ul> | ||
| + | <li>Designed the experiment for testing FA concentration by GCMS</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>June 28:</strong></p> | ||
| + | <ul> | ||
| + | <li>HepG2 cell culturing (first generation)</li> | ||
| + | <li></li> | ||
| + | </ul><br> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div> | <div> | ||
<a href='#' class='head'>Week 1</a> | <a href='#' class='head'>Week 1</a> | ||
| - | <div class='content' align="left"> | + | <div class='content' align="left"> |
| + | <p> </p> | ||
| + | <p><strong>July 2:</strong></p> | ||
| + | <ul> | ||
| + | <li>HepG2 cell passaging</li> | ||
| + | <li>Made sodium palmitate from powder to solution by 50% Ethanol</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 3:</strong></p> | ||
| + | <ul> | ||
| + | <li>Used GCMS to quantify the FA in the medium</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 4:</strong></p> | ||
| + | <ul> | ||
| + | <li>Passaged HepG2 cells</li> | ||
| + | <li>Changed medium for cell line</li> | ||
| + | <li>Searched for information of MTT assay and Transfection by lipofectamine</li> | ||
| + | <li>Got GCMS results for the last day: Not accurate/usable</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 5:</strong></p> | ||
| + | <ul> | ||
| + | <li>Repeated GCMS test again testing FA in medium and we made</li> | ||
| + | <li>Got the result of GCMS: Not accurate/usable</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div> | <div> | ||
<a href='#' class='head'>Week 2</a> | <a href='#' class='head'>Week 2</a> | ||
| - | <div class='content' align="left"> | + | <div class='content' align="left"> |
| + | <p> </p> | ||
| + | <p><strong>July 8:</strong></p> | ||
| + | <ul> | ||
| + | <li>Passaged HepG2 cells</li> | ||
| + | <li>Used some HepG2 cells to extract genomic DNA</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 9:</strong></p> | ||
| + | <ul> | ||
| + | <li>Another try of GCMS with a gradient concentration of FA</li> | ||
| + | <li>Got the result of GCMS: Not match with the concentration we made</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 10:</strong></p> | ||
| + | <ul> | ||
| + | <li>Repeated GCMS: No accurate results</li> | ||
| + | <li>Changed Medium for the cell line</li> | ||
| + | <li>Checked lipofectamine protocol</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 11:</strong></p> | ||
| + | <ul> | ||
| + | <li>GCMS again for the same sodium palmitate we made: Not accurate results</li> | ||
| + | <li>Passaged HepG2 cell line</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p><strong>July 12:</strong></p> | ||
| + | <ul> | ||
| + | <li>Prepared reagents for MTT assay for cell viability test, protocols checked</li> | ||
| + | <li>Changed the medium of the cell line</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 15:40, 19 September 2013
June 2013
Week 4
June 25:
- Prepared DMEM in TC room
- Added FBS (Serum) & Antibiotics into DMEM
June 27:
- Designed the experiment for testing FA concentration by GCMS
June 28:
- HepG2 cell culturing (first generation)
July 2013
Week 1
July 2:
- HepG2 cell passaging
- Made sodium palmitate from powder to solution by 50% Ethanol
July 3:
- Used GCMS to quantify the FA in the medium
July 4:
- Passaged HepG2 cells
- Changed medium for cell line
- Searched for information of MTT assay and Transfection by lipofectamine
- Got GCMS results for the last day: Not accurate/usable
July 5:
- Repeated GCMS test again testing FA in medium and we made
- Got the result of GCMS: Not accurate/usable
Week 2
July 8:
- Passaged HepG2 cells
- Used some HepG2 cells to extract genomic DNA
July 9:
- Another try of GCMS with a gradient concentration of FA
- Got the result of GCMS: Not match with the concentration we made
July 10:
- Repeated GCMS: No accurate results
- Changed Medium for the cell line
- Checked lipofectamine protocol
July 11:
- GCMS again for the same sodium palmitate we made: Not accurate results
- Passaged HepG2 cell line
July 12:
- Prepared reagents for MTT assay for cell viability test, protocols checked
- Changed the medium of the cell line
Week 3
Content 1
Week 4
Content 1
Week 5
Content 1
