Team:Hong Kong HKUST/notebook/mod2
From 2013.igem.org
(Difference between revisions)
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<a href='#' class='head'>Week 4</a> | <a href='#' class='head'>Week 4</a> | ||
- | <div class='content' align="left">Wet lab<br> | + | <div class='content' align="left"> |
- | · Inoculation of pBlueScript KS(+) for training<br> | + | <br>June 24 <br> |
- | · Autoclave basic materials< | + | Wet lab <br> |
- | · Preparing LB<br> | + | · Inoculation of pBlueScript KS(+) for training <br> |
- | · LB-Ampicillin plates poured<br> | + | · Autoclave basic materials <br> |
- | Dry lab<br> | + | · Preparing LB <br> |
- | + | · LB-Ampicillin plates poured | |
+ | Dry lab <br> | ||
+ | Protocols review <br> | ||
+ | <br> | ||
+ | June 25 <br> | ||
+ | Wet lab <br> | ||
+ | · Plasmid extraction of pBlueScript KS(+) <br> | ||
+ | · LB-Chloramphenicol plates poured <br> | ||
+ | Dry lab <br> | ||
+ | · Primers design for FABP1, PPARa, GRP78 <br> | ||
+ | · Informal meeting <br> | ||
+ | <br> | ||
+ | June 26 <br> | ||
+ | Wet lab <br> | ||
+ | · Extraction of gDNA from HepG2 Cells for FABP1 and PPARa <br> | ||
+ | · Transformation of pEGFP-N1 <br> | ||
+ | · Transformation of BBa_K817002 (pFadBA) <br> | ||
+ | <br> | ||
+ | June 27 <br> | ||
+ | Wet lab <br> | ||
+ | · Inoculation of pEGFP-N1 for plasmid extraction <br> | ||
+ | · Inoculation of BBa_K817002 (pFadBA) for plasmid extraction <br> | ||
+ | <br> | ||
+ | June 28 <br> | ||
+ | Wet lab <br> | ||
+ | · Extraction of pEGFP-N1 plasmids by miniprep <br> | ||
+ | · Extraction of BBa_K817002 (pFadBA) <br> | ||
+ | · Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification <br> | ||
+ | · Restriction of c(pFadBA) by EcoR1 and Pst1 <br> | ||
+ | · Ran 0.8% gel for pEGFP-N1 <br> | ||
+ | · Ran 2% gel for BBa_K817002 (pFadBA) <br> | ||
+ | <br> | ||
+ | June 30 <br> | ||
+ | Dry lab <br> | ||
+ | Experiment planning and protocols revision for next week work<br></br> | ||
+ | |||
</div> | </div> | ||
</div> | </div> |
Revision as of 14:35, 19 September 2013
June 2013
Week 4
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work