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Team:Hong Kong HKUST/notebook/mod2
From 2013.igem.org
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<a href='#' class='head'>Week 1</a> | <a href='#' class='head'>Week 1</a> | ||
| - | <div class='content' align="left"> | + | <div class='content' align="left"> |
| + | <br> <b> July 2 </b> <br> | ||
| + | Wet lab <br> | ||
| + | · Digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; , Xba1 and BamH1 for FABP1 <br> | ||
| + | · LB-Chloramphenicol plates (the previous ones were found contaminated) <br> | ||
| + | · Inoculation of pEGFP-N1 for plasmid extraction <br> | ||
| + | · Transformation of pFadBA due chloramphenicol plates contamination <br> | ||
| + | |||
| + | <br> <b> July 3 </b> <br> | ||
| + | · Extraction of pEGFP-N1 plasmids by miniprep <br> | ||
| + | · PCR for PPARa promoter amplification <br> | ||
| + | · Digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; , Xba1 and BamH1 for FABP1 <br> | ||
| + | |||
| + | <br> <b> July 4</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Inoculation of BBa_K817002 pFadBA<br> | ||
| + | · PCR for PPARa promoter cloning from gDNA<br> | ||
| + | · PCR for FABP1 promoter cloning from gDNA<br> | ||
| + | · Ran gel for digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; Xba1 and BamH1 for FABP1; Ase1 and Xhol1 for GRP78<br> | ||
| + | |||
| + | <br> <b> July 5</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Extraction of BBa_K817002 (pFadBA) <br> | ||
| + | · Inoculation of pEGFP-N1<br> | ||
| + | · PCR for PPARa promoter cloning from gDNA<br> | ||
| + | · PCR for FABP1 promoter cloning from gDNA<br> | ||
| + | · Gel check, 0.8% gel for previously gDNA extraction<br> | ||
| + | · Ran gel for PCR check of PPARa<br> | ||
| + | · Ran gel for PCR chack of FABP1<br> | ||
| + | · Poured new LB-Kanamycin plates<br> | ||
| + | · Transformation of BBa_J176171<br> | ||
| + | Dry lab<br> | ||
| + | · Discussion about re-design constructions, possible change for BBa_J176171 as mammalian expression vector<br> | ||
| + | · Primer redesign for PPARa and FABP1<br><br> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 14:44, 19 September 2013
June 2013
Week 4
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
July 2013
Week 1
July 2
Wet lab
· Digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; , Xba1 and BamH1 for FABP1
· LB-Chloramphenicol plates (the previous ones were found contaminated)
· Inoculation of pEGFP-N1 for plasmid extraction
· Transformation of pFadBA due chloramphenicol plates contamination
July 3
· Extraction of pEGFP-N1 plasmids by miniprep
· PCR for PPARa promoter amplification
· Digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; , Xba1 and BamH1 for FABP1
July 4
Wet lab
· Inoculation of BBa_K817002 pFadBA
· PCR for PPARa promoter cloning from gDNA
· PCR for FABP1 promoter cloning from gDNA
· Ran gel for digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; Xba1 and BamH1 for FABP1; Ase1 and Xhol1 for GRP78
July 5
Wet lab
· Extraction of BBa_K817002 (pFadBA)
· Inoculation of pEGFP-N1
· PCR for PPARa promoter cloning from gDNA
· PCR for FABP1 promoter cloning from gDNA
· Gel check, 0.8% gel for previously gDNA extraction
· Ran gel for PCR check of PPARa
· Ran gel for PCR chack of FABP1
· Poured new LB-Kanamycin plates
· Transformation of BBa_J176171
Dry lab
· Discussion about re-design constructions, possible change for BBa_J176171 as mammalian expression vector
· Primer redesign for PPARa and FABP1
July 2
Wet lab
· Digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; , Xba1 and BamH1 for FABP1
· LB-Chloramphenicol plates (the previous ones were found contaminated)
· Inoculation of pEGFP-N1 for plasmid extraction
· Transformation of pFadBA due chloramphenicol plates contamination
July 3
· Extraction of pEGFP-N1 plasmids by miniprep
· PCR for PPARa promoter amplification
· Digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; , Xba1 and BamH1 for FABP1
July 4
Wet lab
· Inoculation of BBa_K817002 pFadBA
· PCR for PPARa promoter cloning from gDNA
· PCR for FABP1 promoter cloning from gDNA
· Ran gel for digestion of pEGFP-N1 by Ase1 and bamH1 for PPARa; Xba1 and BamH1 for FABP1; Ase1 and Xhol1 for GRP78
July 5
Wet lab
· Extraction of BBa_K817002 (pFadBA)
· Inoculation of pEGFP-N1
· PCR for PPARa promoter cloning from gDNA
· PCR for FABP1 promoter cloning from gDNA
· Gel check, 0.8% gel for previously gDNA extraction
· Ran gel for PCR check of PPARa
· Ran gel for PCR chack of FABP1
· Poured new LB-Kanamycin plates
· Transformation of BBa_J176171
Dry lab
· Discussion about re-design constructions, possible change for BBa_J176171 as mammalian expression vector
· Primer redesign for PPARa and FABP1
Week 2
Content 1
Week 3
Content 1
Week 4
Content 1
Week 5
Content 1
