Template:Kyoto/Notebook/Aug 28
From 2013.igem.org
(Difference between revisions)
(→Gel Extraction: (editor:kojima)) |
(→Colony PCR: editor:kojima) |
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===Colony PCR=== | ===Colony PCR=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">No name</span> | ||
+ | {| class="wikitable" | ||
+ | !|Sample||base pair | ||
+ | |- | ||
+ | |8/21 RBS-lysis2-(3)||772 | ||
+ | |- | ||
+ | |8/21 RBS-lysis2-(4)||772 | ||
+ | |- | ||
+ | |8/21 RBS-lysis2-(5)||772 | ||
+ | |- | ||
+ | |8/21 RBS-lysis2-(6)||772 | ||
+ | |} | ||
+ | {| class="wikitable" | ||
+ | !PreDenature||Denature||Annealing||Extension||cycle | ||
+ | |- | ||
+ | |94°C||94°C||55°C||68°C||-- | ||
+ | |- | ||
+ | |5min||30s||30s||48s||30cycles | ||
+ | |} | ||
+ | [[File:igku_Aug19electrophoresis1.png]] | ||
+ | </div> | ||
+ | |||
===Restriction Enzyme Digestion=== | ===Restriction Enzyme Digestion=== | ||
===Electrophoresis=== | ===Electrophoresis=== |
Revision as of 06:14, 21 September 2013
Contents |
Aug 28
Electrophoresis
Miniprep
DNA | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
8/27 Plux | 173.8 | 1.95 | 1.45 |
Ligation
state | Vector | Inserter | ||
---|---|---|---|---|
experiment | 8/71 DT-1 | 3.0 | 8.0 |
- Samples were evaporeted used evaporator into about 3 µL.
sample | MilliQ | Ligation High | total |
---|---|---|---|
3 | 4 | 3.5 | 10.5 |
- incubate one hour at 16 °C
Gel Extraction
Lane | DNA | |
---|---|---|
1 | 100bpladder | -- |
3 | SasA(8/27 Genome PCR production) | |
5 | RpaA(8/27 Genome PCR production) | |
7 | RpaB(8/27 Genome PCR production) | |
9 | PkaiBC(8/27 Genome PCR production) |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Colony PCR
Sample | base pair |
---|---|
8/21 RBS-lysis2-(3) | 772 |
8/21 RBS-lysis2-(4) | 772 |
8/21 RBS-lysis2-(5) | 772 |
8/21 RBS-lysis2-(6) | 772 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 48s | 30cycles |