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Team:Greensboro-Austin/Plasmids and Strains
From 2013.igem.org
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|pSB1C3_Bb_Foward | |pSB1C3_Bb_Foward | ||
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| - | | | + | |TACTAGTAGCGGCCGCTGCAGTC |
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|pSB1cs_Bbprefix_Reverse | |pSB1cs_Bbprefix_Reverse | ||
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| - | | | + | |CTCTAGAAGCGGCCGCGAATTCCAGAAATC |
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|MjYRS_Forward | |MjYRS_Forward | ||
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| - | | | + | |CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG GCACCTGAAGTCAGCCCCAT |
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| Line 236: | Line 236: | ||
|MjYRS_Reverse | |MjYRS_Reverse | ||
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| - | | | + | |CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTA TTCGGAATCTTGCACGCCCT |
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Revision as of 15:45, 17 June 2013
Primer Database
I tried putting the forward and reverse primers next to each other to make life easier. Some of the numbering is a little out out of order just because of the timing that they came in... sorry about that
| Box | Slot | Label | Name | Part | Primer | Source | Template | Homology | For Construction Of | Added by |
|---|---|---|---|---|---|---|---|---|---|---|
| iGEM 2013 Primers | A1 | iGEM001 | fp-151 sfGFP fusion protein forward primer | fp_151_sfGFPP_fus_for | GTT TAA CTT TAA GAA GGA GAT ATA CAT | Kate | ||||
| iGEM 2013 Primers | A2 | iGEM002 | fp-151 sfGFP fusion protein reverse primer | fp151_sfGFP_homo_fus_rev | TTG GTA CTA CGC CAG TAA AGA G | Kate | ||||
| iGEM 2013 Primers | B1 | iGEM003 | pET21_LacI_for_pCCT_homo | AAA ATA AAC AAA TAG GGG TTC CTT TAG AAA AAT AAA CAA ATC GCC CAC CGG AAG GAG CTG | Kate | |||||
| iGEM 2013 Primers | B2 | iGEM004 | pCCT2_rev_pET21_LacI_homo | CAG CTC CTT CCG GTG GGC GAT TTG TTT ATT TTT CTA AAG GAA CCC CTA TTT GTT TAT TTT | Kate | |||||
| iGEM 2013 Primers | D3 | iGEM005 | sfGFP ORF forward primer | sfGFP_ORF_for | GCA TCC AAG GGC GAG GAG C | Kate | ||||
| iGEM 2013 Primers | C2 | iGEM006 | PET21_T7prom_LacI_Rev | CTT CGG TTT GTA CAT ATG TAT ATC TCC TTC TTA AAG TTA A | Kate | |||||
| iGEM 2013 Primers | D1 | iGEM007 | pCCT2 sfGFP ORF forward primer | pCCT2_for_sfGFP_ORF_for | CAC CAC CAC CAC TAA CTG GAA TTC GCG GCC GCT TCT AGA G | Kate | ||||
| iGEM 2013 Primers | D2 | iGEM008 | pCCT2 sfGFP ORF reverse primer | sfGFP_ORF_rev_PCCT2_ | CTC TAG AAG CGG CCG CGA ATT CCA GTT AGT GGT GGT GGT GGT GGT GG | Kate | ||||
| iGEM 2013 Primers | E1 | iGEM009 | pSB1C3 Bb forward primer | pSB1C3_Bb_Foward | TACTAGTAGCGGCCGCTGCAGTC | Kate | ||||
| iGEM 2013 Primers | E2 | iGEM010 | pSB1C3 Bb reverse primer | pSB1cs_Bbprefix_Reverse | CTCTAGAAGCGGCCGCGAATTCCAGAAATC | Kate | ||||
| iGEM 2013 Primers | F1 | iGEM011 | BB_prefix_for_tyrosinase | ACT ACA CCT TCG ACG TCT GAC TGG AAT TCG CGG CCG CTT C | Kate | |||||
| iGEM 2013 Primers | F2 | iGEM012 | tyrosinase_rev_BB_prefix | GAA GCG GCC GCG AAT TCC AGT CAG ACG TCG AAG GTG TAG T | Kate | |||||
| iGEM 2013 Primers | G1 | MJH 197 | pSB1C3_bb_for_MjYRS ca | Kate | ||||||
| iGEM 2013 Primers | G2 | MJH 198 | MjYRS_cass_rev_pSB1C3 | Kate | ||||||
| iGEM 2013 Primers | C1 | iGEM017 | pET21_T7promRBS_rev | ATG TAT ATC TCC TTC TTA AAG TTA AAC | Kate | |||||
| iGEM 2013 Primers | H1 | iGEM018 | MjYRS_Forward | CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG GCACCTGAAGTCAGCCCCAT | Kate | |||||
| iGEM 2013 Primers | H2 | iGEM019 | MjYRS_Reverse | CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTA TTCGGAATCTTGCACGCCCT | Kate | |||||
| iGEM 2013 Primers | I1 | iGEM020 | ORF438_for_pET_T7pr | Kate |
|}
Plasmid Database
| Box | Slot | Label | Name | Part | Plasmid | Source | Template | Homology | For Construction Of | Added by |
|---|---|---|---|---|---|---|---|---|---|---|
| iGEM 2013 Plasmid | A1 | iGEM 013 | pSB1C3_synthetase_cas_ | Kate | ||||||
| iGEM 2013 Plasmid | A2 | iGEM 014 | pSB1C3_Synthetase_Cass | Kate | ||||||
| iGEM 2013 Plasmid | A3 | iGEM 015 | pSB1C3_Synthetase_Cas | Kate | ||||||
| iGEM 2013 Plasmid | A4 | iGEM 016 | pSB1C3_Synthetase_Cass | Kate |
