Template:Kyoto/Notebook/Aug 30

From 2013.igem.org

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Revision as of 08:09, 22 September 2013

Contents

Aug 30

Gel Extraction

No Name

Namequantityconcentration[µg/mL]260/280260/230
PT181 atte(2) Ets305.3 2.2 1.66 0.36
PT181 atte(2) Xtp217.7 3.9 1.73 0.04
PT181 anti(2) Ets347.7 3.1 1.88 0.31
PT181 anti(2) Xtp376.5 5.9 0.49 2.36
PSBIC3 Ets436.6 30.7 1.03 1.28
PSBIC3 Xtp457.4 7.9 1.65 0.42
Spinach Ets490.5 3.0 1.87 0.26
tetR aptamer 12-1R Ets451.7 -12.8 5.56 -0.04

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
RBS-lysis2 9264.6　1.97　2.21
RBS-lysis2 12220.0　1.89　2.46
RBS-lysis2 14195.3　1.88　1.53

Colony PCR

No name

Samplebase pair
8/29 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(8/20 LB Amp) (1)~(4)2143
8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)1227
8/29 Pbad-larac-RBS-RFP{BBaI13516} (8/21 LB-CP) (1)~(2)2256
8/29 RBS-lysis3-DT-(1)1210
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min　30s　30s　2min　8min
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