Template:Kyoto/Notebook/Aug 30

(Difference between revisions)

Revision as of 09:45, 22 September 2013

No Name

Name	quantity	concentration[µg/mL]	260/280	260/230
pT181 attenuator(2) (EcoRI & SpeI)	305.3	2.2	1.66	0.36
pT181 attenuator(2) (XbaI & PstI)	217.7	3.9	1.73	0.04
pT181 antisense(2) (EcoRI & SpeI)	347.7	3.1	1.88	0.31
pT181 antisense(2) (XbaI & PstI)	376.5	5.9	0.49	2.36
pSB1C3 (EcoRI & SpeI)	436.6	30.7	1.03	1.28
pSB1C3 (XbaI & PstI)	457.4	7.9	1.65	0.42
Spinach (EcoRI & SpeI)	490.5	3.0	1.87	0.26
tetR aptamer 12_1R (EcoRI & SpeI)	451.7	-12.8	5.56	-0.04

No name

DNA	concentration[µg/mL]	260/280	260/230
RBS-lysis2 9	264.6	1.97	2.21
RBS-lysis2 12	220.0	1.89	2.46
RBS-lysis2 14	195.3	1.88	1.53

No name

Sample	base pair
8/29 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(8/20 LB Amp) (1)~(4)	2143
8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)	1227
8/29 Pbad/araC-RBS-RFP{BBa_I13516} (8/21 LB-CP) (1)~(2)	2256
8/29 RBS-lysis3-DT-(1)	1210

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	2min	30cycle

No name

No name

	8/30 RBS-lysis2 (9)	EcoRI	SpeI	10x bufferB	100x BSA	MilliQ	total
2 cuts	7.5 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	18.2 µL	30 µL
NC	0.5 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/22 pSB1C3-(2)	EcoRI	SpeI	10x Buffer	100x BSA	MilliQ	total
2 cuts	8 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	17.7 µL	30 µL
NC	1 µL	0 µL	0 µL	1 µL	0.1 µL	7.9 µL	10 µL

	8/22 pSB1C3-(2)	XbaI	PstI	BufferD	BSA	MilliQ	total
2 cuts	8 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	17.7 µL	30 µL
NC	1 µL	0 µL	0 µL	1 µL	0.1 µL	7.9 µL	10 µL

	8/21 tRNA-Spinach (1)	EcoRI	SpeI	10x BufferB	100x BSA	MilliQ	total
2 cuts	4 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	21.9 µL	30 µL
NC	0.5 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/21 tetR aptamer 12_1R (1)	EcoRI	SpeI	10xBufferB	100xBSA	MilliQ	total
2 cuts	3 µL	0.5 µL	0.5 µL	3 µL	0.3 µL	22.7 µL	30 µL
NC	0.4 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/21 pT181 attenuator-(2)	EcoRI	SpeI	XbaI	PstI	10x BufferB	10x BufferD	100x BSA	MilliQ	total
2 cuts	4 µL	0.5 µL	0.5 µL	0 µL	0 µL	3 µL	0 µL	0.3 µL	21.7 µL	30 µL
NC	0.5 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0 µL	0.1 µL	8.4 µL	10 µL
2 cuts	4 µL	0 µL	0 µL	0.5 µL	0.5 µL	0 µL	3 µL	0.3 µL	21.7 µL	30 µL
NC	0.5 µL	0 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0.1 µL	8.4 µL	10 µL

	8/21 pT181 antisense-(2)	EcoRI	SpeI	XbaI	PstI	10x BufferB	10x BufferD	100x BSA	MilliQ	total
2 cuts	9 µL	0.5 µL	0.5 µL	0 µL	0 µL	3 µL	0 µL	0.3 µL	16.7 µL	30 µL
NC	1 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0 µL	0.1 µL	7.9 µL	10 µL
2 cuts	9 µL	0 µL	0 µL	0.5 µL	0.5 µL	0 µL	3 µL	0.3 µL	16.7 µL	30 µL
NC	1 µL	0 µL	0 µL	0 µL	0 µL	0 µL	1 µL	0.1 µL	7.9 µL	10 µL

@@ Line 47: / Line 47: @@
 |8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)||1227
 |-
-|8/29 Pbad-larac-RBS-RFP{BBaI13516} (8/21 LB-CP) (1)~(2)||2256
+|8/29 Pbad/araC-RBS-RFP{BBa_I13516} (8/21 LB-CP) (1)~(2)||2256
 |-
 |8/29 RBS-lysis3-DT-(1)||1210
@@ Line 54: / Line 54: @@
 !PreDenature||Denature||Annealing||Extension||cycle
 |-
-|　94&deg;C||　94&deg;C||　55&deg;C||　68&deg;C||--
+|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 |-
-|5min　||30s　||30s　||2min　||30cycle
+|5min||30s||30s||2min||30cycle
 |}
 </div>
 ===Ethanol Precipitation===
 <div class="experiment">