Team:Groningen/outreach/summerschool

From 2013.igem.org

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<h2>iGEM summerschool 2013:</h2>
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<h2>iGEM summer school 2013:</h2>
This years iGEM team organized a three day summer school. The idea was to stay as close to our subject as possible. So our main topics: secretion, biofilm production passed by during this week. The high school students started the week with a short introduction about iGEM and a tour through our building, the Linnausborg. They isolated the plasmid of three different strains: a wildtype amylase producing strain, a knock out of this amylase and an amylase secretion mutant. During the week they had to discover which phenotypes belonged to which strains and of course to the corresponding numbers they got. To do so, they did a halo test and a restriction analysis. The halo test showed the difference between the wildtype strain and both mutant strains, and the restriction analysis the difference between both mutant strains. They also had the opportunity to grow a biofilm and to do an antibiotic resistance test. To get an idea of how the strain reacts to different antibiotics. During the week they had a lecture about Bacillus at the UMCG and a discussion with some employees of the university to get an idea of the work of a scientist. After working in a molecular biology lab for a week they all received a certificate.
This years iGEM team organized a three day summer school. The idea was to stay as close to our subject as possible. So our main topics: secretion, biofilm production passed by during this week. The high school students started the week with a short introduction about iGEM and a tour through our building, the Linnausborg. They isolated the plasmid of three different strains: a wildtype amylase producing strain, a knock out of this amylase and an amylase secretion mutant. During the week they had to discover which phenotypes belonged to which strains and of course to the corresponding numbers they got. To do so, they did a halo test and a restriction analysis. The halo test showed the difference between the wildtype strain and both mutant strains, and the restriction analysis the difference between both mutant strains. They also had the opportunity to grow a biofilm and to do an antibiotic resistance test. To get an idea of how the strain reacts to different antibiotics. During the week they had a lecture about Bacillus at the UMCG and a discussion with some employees of the university to get an idea of the work of a scientist. After working in a molecular biology lab for a week they all received a certificate.

Revision as of 18:59, 22 September 2013

iGEM summer school 2013:

This years iGEM team organized a three day summer school. The idea was to stay as close to our subject as possible. So our main topics: secretion, biofilm production passed by during this week. The high school students started the week with a short introduction about iGEM and a tour through our building, the Linnausborg. They isolated the plasmid of three different strains: a wildtype amylase producing strain, a knock out of this amylase and an amylase secretion mutant. During the week they had to discover which phenotypes belonged to which strains and of course to the corresponding numbers they got. To do so, they did a halo test and a restriction analysis. The halo test showed the difference between the wildtype strain and both mutant strains, and the restriction analysis the difference between both mutant strains. They also had the opportunity to grow a biofilm and to do an antibiotic resistance test. To get an idea of how the strain reacts to different antibiotics. During the week they had a lecture about Bacillus at the UMCG and a discussion with some employees of the university to get an idea of the work of a scientist. After working in a molecular biology lab for a week they all received a certificate.


Stukje geschreven door Kim en Amarins:

Deze zomer organiseerde iGEM een summer school voor middelbare scholieren. Hieraan hebben wij, Kim en Amarins, meegedaan. Onze scheikundeleraar heeft ons aanbevolen mee te doen, omdat dit nuttig zou kunnen zijn voor ons profielwerkstuk. Deze drie daagse kennismaking met moleculaire biologie en de Rijksuniversiteit Groningen was inderdaad interessant. We hebben namelijk onderzoek gedaan naar stammen van Bacillus subtilis. De eerste dag hebben we een rondleiding gehad door de Linnaeusborg, waarbij we ook het lab van iGEM hebben gezien en informatie kregen over het onderzoeksteam. Hierna vertrokken we naar ons eigen lab voor de komende dagen. Daar hebben we een aantal proeven gedaan waarbij we onder andere plasmide hebben geïsoleerd en restrictie analyse hebben gedaan. Het was leuk om met nieuwe apparatuur te werken en de basisvaardigheden te oefenen. We zijn op de tweede dag met z’n allen naar het UMCG geweest en hier kregen we een praatje van prof. dr. Jan Maarten van Dijl, en we konden een kijkje nemen in zijn ML-2 laboratorium. Donderdag, de laatste dag van deze summer school, hebben we onder andere geluncht met onderzoekers en onze experimenten afgerond. Ook kregen we een certificaat en was er een borrel. Uiteindelijk zijn we blij dat we hebben meegedaan en was het gezellig en wel inspirerend voor ons profielwerkstuk. Verder hebben we een goede indruk gekregen van studeren in Groningen.