Template:Kyoto/Notebook/Sep 1

(Difference between revisions)

Revision as of 00:21, 23 September 2013

No name

Name	Sample	Competent Cells	Total	Plate
8/31 tRNA-Spinach(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 antisense(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 attenuator(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 antisense(pSB1C3)	2µL	20µL	22µL	CP
8/31 pT181 attenuator(2)-DT	2µL	20µL	22µL	CP
8/31 pT181 antisense-DT	2µL	20µL	22µL	CP
8/31 tRNA-Spniach-DT	2µL	20µL	22µL	CP
8/31 RBS-lysis2-DT	2µL	20µL	22µL	CP
8/31 Pconst-RBS-tetR-DT	2µL	20µL	22µL	Amp
8/31 Plac-RBS-lacZα-DT	2µL	20µL	22µL	CP
8/31 Ptet-RBS-lacZα-DT	2µL	20µL	22µL	CP
8/31	2µL	20µL	22µL	CP

No name

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	30min	30cycle

@@ Line 33: / Line 33: @@
 </div>
-===PCR===
+===ligation production PCR===
 <div class="experiment">
+<span class="author">No name</span>
+{| class="wikitable"
+!Sample||base pair
+|-
+|8/31 tRNA Spinach(pSB1C3)||459
+|-
+|8/31 pT181 antisense(2)(pSB1C3)(E+S)||415
+|-
+|8/31 pT181 attenuator(2)(pSB1C3)(X+P)||601
+|-
+|8/31 pT181 antisense(2)(pSB1C3)(X+P)||415
+|-
+|8/31 pT181 attenuator(2)-DT||754
+|-
+|8/31 pT181 antisense(2)-DT||577
+|-
+|8/31 tRNA Spinach-DT||596
+|-
+|8/22 pSB1C3(2)(controll)||1383
+|}
+{| class="wikitable"
+!PreDenature||Denature||Annealing||Extension||cycle
+|-
+|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
+|-
+|5min||30s||30s||30min||30cycle
+|}
 </div>
 ===Master Plate===
 <div class="experiment">