Team:Glendale CC AZ/Protocols/GrowthCurve

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==== E.coli Bacterial Growth Curve Assay Protocol ====
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/* ================ The Timeline ================ */
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====Materials====
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    * 1 brain-heart infusion (BHI) broth culture of 5h E.coli
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    * 1 tube with 5.0 ml BHI broth
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    * Spectrophotometric tubes
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    * Plastic disposable pipettes
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    * Spectrophotometer at 590 nm 37°C water bath
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====Procedures====
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  1.  Add approximately 6 drops of the BHI into a spectrophotometer tube; this will be the blank tube.
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  2. Add about 6 drops of BHI into a spectrophotometer tube and and another 6 drops of the E.coli broth into the same tube; this will be experimental tube.
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  3. Read the blank tube on the spectrophotometer and blank the system at 590 nm.
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  4. Read the experimental tube at 590 nm and record the data.
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  5. Incubate the experimental tube for 20 minutes at 37°C.
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  6. Once the incubation is complete, read the experimental tube on the spectrophotometer and record the data.
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  7. Repeat steps 5-6 as many times as desired to get a good growth curve.
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  8. Graph the data accordingly.
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<h4>Glendale Community College Arizona<img
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  <h5><p>Protocols</p><img style="border: 0px solid ; width: 400px; height: 200px;" alt="iGEM" src="https://static.igem.org/mediawiki/igem.org/1/19/Tubes2GCC.JPG" align="right">
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve  Assay</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/NaCl">NaCl Growth Curve Assay</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/SurivalGrowth">Survival Growth  Assay</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/RestrictionDigest">Restriction Digest</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/DNAIsolation">DNA Isolation</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Bioinformatics">Bioinformatics</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation">Ligation</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Transformation">Transformation</a></p>
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<p><b>E.coli Bacterial Growth Curve Assay Protocol </p></b>
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<p> Materials </p>
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<ul><li>1 brain-heart infusion (BHI) broth culture of 5h E.coli
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</li><li>1 tube with 5.0 ml BHI broth
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</li><li>Spectrophotometric tubes
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37°C water bath</li></ul>
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<p>Procedures </p>
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<ol><li>Add approximately 6 drops of the BHI into a spectrophotometer tube; this will be the blank tube.</li><li>Add about 6 drops of BHI into a spectrophotometer tube and and another 6 drops of the E.coli broth into the same tube; this will be experimental tube.</li><li>Read the blank tube on the spectrophotometer and blank the system at 590 nm.</li><li>Read the experimental tube at 590 nm and record the data.</li><li>Incubate the experimental tube for 20 minutes at 37°C.</li><li>Once the incubation is complete, read the experimental tube on the spectrophotometer and record the data.</li><li>Repeat steps 5-6&nbsp; as many times as desired to get a good growth curve.</li><li>Graph the data accordingly.</li></ol>
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Revision as of 02:20, 23 September 2013

E.coli Bacterial Growth Curve Assay Protocol

Materials

   * 1 brain-heart infusion (BHI) broth culture of 5h E.coli
   * 1 tube with 5.0 ml BHI broth
   * Spectrophotometric tubes
   * Plastic disposable pipettes
   * Spectrophotometer at 590 nm 37°C water bath

Procedures

  1.  Add approximately 6 drops of the BHI into a spectrophotometer tube; this will be the blank tube.
  2. Add about 6 drops of BHI into a spectrophotometer tube and and another 6 drops of the E.coli broth into the same tube; this will be experimental tube.
  3. Read the blank tube on the spectrophotometer and blank the system at 590 nm.
  4. Read the experimental tube at 590 nm and record the data.
  5. Incubate the experimental tube for 20 minutes at 37°C.
  6. Once the incubation is complete, read the experimental tube on the spectrophotometer and record the data.
  7. Repeat steps 5-6  as many times as desired to get a good growth curve.
  8. Graph the data accordingly.
Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve"