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Team:Glendale CC AZ/Protocols/SurivalGrowth
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Revision as of 03:00, 23 September 2013
Glendale Community College Arizona
Protocols
Growth Curve Assay
NaCl Growth Curve Assay
Survival Growth Assay
Alkaline Lysis Plasmid Miniprep
Restriction Digest
DNA Isolation
Bioinformatics
Ligation
Transformation
Survival Growth Assay
Materials
-P200 micropipette -10µl bacteria -90µl LB media -Agar plates with appropriate antibiotic -Spreader -20mL ethanol (to sterilize spreader) -Incubator set to 37°C.
Procedure
1. Label all plates (Keep upside down to avoid condensation on agar)
2. Pipette LB media into tube containing bacteria; aspirate.
3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.
4. Briefly flame the spreader and let cool momentarily.
5. Spread bacteria-LB solution all over plate.
6. Incubate at 37°C for up to 24 hours.
