Team:Buenos Aires/ resqrfp

From 2013.igem.org

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(mRFP Quantification)
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= mRFP Quantification =
= mRFP Quantification =
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Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.
Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.
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[[File:pArsrfp.jpg]]
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Revision as of 01:42, 24 September 2013

mRFP Quantification

To Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.

File:PArsrfp.jpg