Template:Kyoto/Notebook/Sep 2

From 2013.igem.org

(Difference between revisions)
(→Liquid Culture)
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!Sample||medium
!Sample||medium
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|-
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|8/21 pSB1C3(BBa_J04450)(1)||Plusgrow medium(+CP)
+
|9/1 entA-(Master Plate)-1||4mL SOB(+Km)
-
|-
+
37°C
-
|8/21 pSB1C3(BBa_J04450)(2)||Plusgrow medium(+CP)
+
 
-
|-
+
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|8/18 RBS-lysis3-DT||Plusgrow medium(+CP)
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== Headline text ==
== Headline text ==

Revision as of 05:09, 23 September 2013

Contents

Sep 2

Liquid Culture

No name

Samplemedium
9/1 entA-(Master Plate)-14mL SOB(+Km)

37°C

Headline text

  • at the same time made maste plate.
  • incubate 37°C 8hour.

Colony PCR

No name

Restriction Enzyme Digestion

Nakamoto

Liquid Culture

Tatsui,Stephane,Nakamoto

Master Plate

No name

Gel Extraction

EDTA Solution

Hirano

Colony PCR

Hirano

Master Plate

Hirano

Liquid Culture

Hirano

Colony PCR

Hirano

Transformation

Hirano
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