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Template:Kyoto/Notebook/Aug 16
From 2013.igem.org
(Difference between revisions)
(→Aug 16) |
(→Colony PCR) |
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==Aug 16== | ==Aug 16== | ||
| + | 8/15 | ||
| + | BBa_R0061(Plax) | ||
| + | BBa_I13504(RBS-GFP-DT))(1) | ||
| + | BBa_I13504(RBS-GFP-DT)(2) | ||
| + | BBa_I0462(RBS-luxR-DT)(1) | ||
| + | BBa_I0462(RBS-luxR-DT)(2) | ||
| + | BBa_K117000(lysis1)(1) | ||
| + | BBa_K117000(lysis1)(2) | ||
| + | BBa_K748002(lysis3)(1) | ||
| + | BBa_K748002(lysis3)(2) | ||
| + | BBa_B0015(DT) | ||
| + | BBa_I732020(RBS-laczα-DT) | ||
| + | |||
| + | |||
| + | |||
===Colony PCR=== | ===Colony PCR=== | ||
<div class="experiment"> | <div class="experiment"> | ||
| Line 6: | Line 21: | ||
!Sample||base pair | !Sample||base pair | ||
|- | |- | ||
| - | |( | + | |8/15 BBa_R0061(Plax)||344 |
| + | |- | ||
| + | |8/15 BBa_I13504(RBS-GFP-DT))(1)||1174 | ||
| + | |- | ||
| + | |8/15 BBa_I13504(RBS-GFP-DT)(2)||1174 | ||
| + | |- | ||
| + | |8/15 BBa_I0462(RBS-luxR-DT)(1)||1174 | ||
| + | |- | ||
| + | |8/15 BBa_I0462(RBS-luxR-DT)(2)||1174 | ||
| + | |- | ||
| + | |8/15 BBa_K117000(lysis1)(1)||456 | ||
| + | |- | ||
| + | |8/15 BBa_K117000(lysis1)(2)||456 | ||
| + | |- | ||
| + | |8/15 BBa_K748002(lysis3)||997 | ||
| + | |- | ||
| + | |8/15 BBa_B0015(DT)||443 | ||
| + | |- | ||
| + | |8/15 BBa_I732020(RBS-laczα-DT)||703 | ||
| + | |- | ||
| + | |negative control||-- | ||
|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
| Line 16: | Line 51: | ||
|} | |} | ||
</div> | </div> | ||
| - | + | ||
| - | + | ||
{| class="wikitable" | {| class="wikitable" | ||
!Sample||base pair | !Sample||base pair | ||
| Line 30: | Line 64: | ||
|（◎Д◎） || || || || | |（◎Д◎） || || || || | ||
|} | |} | ||
| - | |||
===Master Plate=== | ===Master Plate=== | ||
Revision as of 13:52, 23 September 2013
Contents |
Aug 16
8/15 BBa_R0061(Plax) BBa_I13504(RBS-GFP-DT))(1) BBa_I13504(RBS-GFP-DT)(2) BBa_I0462(RBS-luxR-DT)(1) BBa_I0462(RBS-luxR-DT)(2) BBa_K117000(lysis1)(1) BBa_K117000(lysis1)(2) BBa_K748002(lysis3)(1) BBa_K748002(lysis3)(2) BBa_B0015(DT) BBa_I732020(RBS-laczα-DT)
Colony PCR
| Sample | base pair |
|---|---|
| 8/15 BBa_R0061(Plax) | 344 |
| 8/15 BBa_I13504(RBS-GFP-DT))(1) | 1174 |
| 8/15 BBa_I13504(RBS-GFP-DT)(2) | 1174 |
| 8/15 BBa_I0462(RBS-luxR-DT)(1) | 1174 |
| 8/15 BBa_I0462(RBS-luxR-DT)(2) | 1174 |
| 8/15 BBa_K117000(lysis1)(1) | 456 |
| 8/15 BBa_K117000(lysis1)(2) | 456 |
| 8/15 BBa_K748002(lysis3) | 997 |
| 8/15 BBa_B0015(DT) | 443 |
| 8/15 BBa_I732020(RBS-laczα-DT) | 703 |
| negative control | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| °C | °C | °C | °C | -- |
| （◎Д◎） |
| Sample | base pair |
|---|---|
| (｀・ω・´) |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| °C | °C | °C | °C | -- |
| （◎Д◎） |
Master Plate
| Number | Use LB plate (+抗生物質) |
|---|---|
| ʅ（´◔౪◔）ʃ |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| いでんし | なんちゃらµL | ごにょごにょµL | ほにゃららµL | こーせーぶっしつ |
| いかどうよう | ||||
Liquid Culture
| Sample | medium |
|---|---|
| (・ω・) | |
| （・Ｘ・） |
