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Template:Kyoto/Notebook/Sep 12
From 2013.igem.org
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|Fusion1atte(pSB1c3)||172.0 ||1.96 ||2.31 | |Fusion1atte(pSB1c3)||172.0 ||1.96 ||2.31 | ||
| - | | | + | |- |
|RBS-lisis1-DT||235.1||1.59||1.60 | |RBS-lisis1-DT||235.1||1.59||1.60 | ||
| - | | | + | |- |
| RBS-lisis2-DT||387.7||1.91||2.01 | | RBS-lisis2-DT||387.7||1.91||2.01 | ||
| - | | | + | |- |
| RBS-lisis3-DT||387.9||1.91||2.09 | | RBS-lisis3-DT||387.9||1.91||2.09 | ||
| - | | | + | |- |
|Plux||166.7||1.98||1.63 | |Plux||166.7||1.98||1.63 | ||
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</div> | </div> | ||
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===Electrophoresis=== | ===Electrophoresis=== | ||
<div class="experiment"> | <div class="experiment"> | ||
Revision as of 13:35, 23 September 2013
Contents |
Sep 12
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181atte-DT | 293.6 | 1.88 | 1.84 |
| Pcon-apt12-1R-DT | 303.8 | 1.75 | 1.64 |
| Fusion1atte(pSB1c3) | 172.0 | 1.96 | 2.31 |
| RBS-lisis1-DT | 235.1 | 1.59 | 1.60 |
| RBS-lisis2-DT | 387.7 | 1.91 | 2.01 |
| RBS-lisis3-DT | 387.9 | 1.91 | 2.09 |
| Plux | 166.7 | 1.98 | 1.63 |
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | 9/11 P-RBS-lisis1-DT(Colony PCR prodution) |
Liquid Culture
| Sample | medium |
|---|---|
| 9/10 Pcon-apt12-1R-DT1 | Plusgrow medium(+Amp) |
| 9/10 DT-Pcon-pT181atte1 | Plusgrow medium(+CP) |
| 9/10 Fusion1-atte(pSB1c3) | Plusgrow medium(+CP) |
Electrophoresis
1
| Lane | Sample | ||||
|---|---|---|---|---|---|
| 1 | 1kb ladder | ||||
| 2 | - | 3 | pSB4K5 NC(EcoR1&Spe1) | ||
| 4 | - | 5 | - | 6 | Pcon-RBS-lacz-DT( EcoR1&Spe1) |
| 7 | - | 8 | 1kb ladder |
| Lane | Sample | ||||
|---|---|---|---|---|---|
| 1 | 1kb ladder | ||||
| 2 | - | 3 | Ptet-pT181anti NC(Spe1&Pst1) | ||
| 4 | - | 5 | - | 6 | -- |
| 7 | 8 | 1kb ladder |
