Template:Kyoto/Notebook/Aug 17

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(Difference between revisions)
(→Aug 19)
(→Aug 17)
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===Aug 17===
===Aug 17===
 +
===Colony PCR===
 +
<div class="experiment">
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<span class="author">Nakamoto</span>
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{| class="wikitable"
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!|Sample||base pair
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|-
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|8/15 BBa_K112806(lysis2) -(1)||828
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|-
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|negative control||--
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|}
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{| class="wikitable"
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!PreDenature||Denature||Annealing||Extension||cycle
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|-
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|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
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|5min||30s||30s||1min||30cycles
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|}
 +
<!-- [[File:image]] -->
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|8/10 BBa_Q0400 -(1)||3995
 +
|-
 +
|negative control||--
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||30s||30cycles
 +
|}
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[[File:igku_Aug19electrophoresis2.png]]
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</div>
 +
<br>
===Colony PCR===
===Colony PCR===

Revision as of 13:35, 23 September 2013

Aug 17

Colony PCR

Nakamoto

Samplebase pair
8/15 BBa_K112806(lysis2) -(1)828
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/10 BBa_Q0400 -(1)3995
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Igku Aug19electrophoresis2.png


Colony PCR

Nakamoto

Samplebase pair
8/15 BBa_K112806(lysis2) -(1)828
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/10 BBa_Q0400 -(1)3995
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Igku Aug19electrophoresis2.png


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