Template:Kyoto/Notebook/Aug 17
From 2013.igem.org
(Difference between revisions)
(→Colony PCR) |
(→Colony PCR) |
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|5min||30s||30s||1min||30cycles | |5min||30s||30s||1min||30cycles | ||
|} | |} | ||
- | + | [[File:image]] | |
{| class="wikitable" | {| class="wikitable" | ||
!Sample||base pair | !Sample||base pair | ||
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|5min||30s||30s||30s||30cycles | |5min||30s||30s||30s||30cycles | ||
|} | |} | ||
- | + | [[File:image]] | |
+ | </div> | ||
+ | |||
+ | ===Liquid Culture=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">Nakamoto</span> | ||
+ | {| class="wikitable" | ||
+ | !Sample||medium | ||
+ | |- | ||
+ | |8/15 BBa_K112806(lysis2) -(1)||Plusgrow medium(+CP) | ||
+ | |- | ||
+ | |8/15 BBa_Q04400(tetR) -(1)||Plusgrow medium(+Amp) | ||
+ | |- | ||
+ | |} | ||
</div> | </div> |
Revision as of 13:58, 23 September 2013
Aug 17
Colony PCR
Sample | base pair |
---|---|
8/15 BBa_K112806(lysis2) -(1) | 828 |
negative control | -- |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 1min | 30cycles |
Sample | base pair |
---|---|
8/10 BBa_Q0400 -(1) | 3995 |
negative control | -- |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 30s | 30cycles |
Liquid Culture
Sample | medium |
---|---|
8/15 BBa_K112806(lysis2) -(1) | Plusgrow medium(+CP) |
8/15 BBa_Q04400(tetR) -(1) | Plusgrow medium(+Amp) |