Team:Greensboro-Austin/Plasmids and Strains

From 2013.igem.org

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Revision as of 23:34, 19 June 2013

Primer Database

I tried putting the forward and reverse primers next to each other to make life easier. Some of the numbering is a little out out of order just because of the timing that they came in... sorry about that

Box Slot Label Name Part Primer Source Template Homology For Construction Of Added by
iGEM 2013 Primers A1 iGEM001 fp-151 sfGFP fusion protein forward primer fp_151_sfGFPP_fus_for GTT TAA CTT TAA GAA GGA GAT ATA CAT Kate
iGEM 2013 Primers A2 iGEM002 fp-151 sfGFP fusion protein reverse primer fp151_sfGFP_homo_fus_rev TTG GTA CTA CGC CAG TAA AGA G Kate
iGEM 2013 Primers B1 iGEM003 pET21_LacI_for_pCCT_homo AAA ATA AAC AAA TAG GGG TTC CTT TAG AAA AAT AAA CAA ATC GCC CAC CGG AAG GAG CTG Kate
iGEM 2013 Primers B2 iGEM004 pCCT2_rev_pET21_LacI_homo CAG CTC CTT CCG GTG GGC GAT TTG TTT ATT TTT CTA AAG GAA CCC CTA TTT GTT TAT TTT Kate
iGEM 2013 Primers D3 iGEM005 sfGFP ORF forward primer sfGFP_ORF_for GCA TCC AAG GGC GAG GAG C Kate
iGEM 2013 Primers C2 iGEM006 PET21_T7prom_LacI_Rev CTT CGG TTT GTA CAT ATG TAT ATC TCC TTC TTA AAG TTA A Kate
iGEM 2013 Primers D1 iGEM007 pCCT2 sfGFP ORF forward primer pCCT2_for_sfGFP_ORF_for CAC CAC CAC CAC TAA CTG GAA TTC GCG GCC GCT TCT AGA G Kate
iGEM 2013 Primers D2 iGEM008 pCCT2 sfGFP ORF reverse primer sfGFP_ORF_rev_PCCT2_ CTC TAG AAG CGG CCG CGA ATT CCA GTT AGT GGT GGT GGT GGT GGT GG Kate
iGEM 2013 Primers E1 iGEM009 pSB1C3 Bb forward primer pSB1C3_Bb_Foward TACTAGTAGCGGCCGCTGCAGTC Kate
iGEM 2013 Primers E2 iGEM010 pSB1C3 Bb reverse primer pSB1cs_Bbprefix_Reverse CTCTAGAAGCGGCCGCGAATTCCAGAAATC Kate
iGEM 2013 Primers F1 iGEM011 BB_prefix_for_tyrosinase ACT ACA CCT TCG ACG TCT GAC TGG AAT TCG CGG CCG CTT C Kate
iGEM 2013 Primers F2 iGEM012 tyrosinase_rev_BB_prefix GAA GCG GCC GCG AAT TCC AGT CAG ACG TCG AAG GTG TAG T Kate
iGEM 2013 Primers G1 MJH 197 pSB1C3_bb_for_MjYRS ca Kate
iGEM 2013 Primers G2 MJH 198 MjYRS_cass_rev_pSB1C3 Kate
iGEM 2013 Primers C1 iGEM017 pET21_T7promRBS_rev ATG TAT ATC TCC TTC TTA AAG TTA AAC Kate
iGEM 2013 Primers H1 iGEM018 MjYRS_Forward CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG GCACCTGAAGTCAGCCCCAT Kate
iGEM 2013 Primers H2 iGEM019 MjYRS_Reverse CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTA TTCGGAATCTTGCACGCCCT Kate
iGEM 2013 Primers I1 iGEM020 ORF438_for_pET_T7pr Kate
iGEM 2013 Primers I2 iGEM021 RS_internal_seq_for CCCGGTTTTAACGGGTCTGG Razan
iGEM 2013 Primers J1 iGEM022 RS_5'_seq_for CTCAAGAAGATCATCTTATTAATCAG Razan

Plasmid Database

Box Slot Label Name Part Plasmid Source Template Homology For Construction Of Added by
iGEM 2013 Plasmid A1 iGEM 013 pSB1C3_synthetase_cas_ Kate
iGEM 2013 Plasmid A2 iGEM 014 pSB1C3_Synthetase_Cass Kate
iGEM 2013 Plasmid A3 iGEM 015 pSB1C3_Synthetase_Cas Kate
iGEM 2013 Plasmid A4 iGEM 016 pSB1C3_Synthetase_Cass Kate
Retrieved from "http://2013.igem.org/Team:Greensboro-Austin/Plasmids_and_Strains"