From 2013.igem.org
(Difference between revisions)
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| - | |}
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| - | ===PCR===
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| - | <!-- ここから -->
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| - | <div class="experiment">
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| - | <span class="author">No name</span>
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| - | {| class="wikitable"
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| - | !genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
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| - | |-
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| - | | ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
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| - | |}
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| - | {| class="wikitable"
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| - | !PreDenature||Denature||Annealing||Extension||cycle
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| - | |-
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| - | | °C|| °C|| °C|| °C||--
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| - | |-
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| - | |（◎Д◎） || || || ||
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| | |} | | |} |
Revision as of 15:02, 23 September 2013
Sep 8
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
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| （´ω｀） | | | |
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| （ΦωΦ^） | | | |
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File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| （´ω｀） | | | |
|
| （ΦωΦ^） | | | |
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File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| （´ω｀） | | | |
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| （ΦωΦ^） | | | |
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Ligation
じっけんしゃ
| state | Vector | Inserter | Ligation High ver.2
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| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL
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| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL
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Restriction Enzyme Digestion
じっけんしゃ
| | DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total
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| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL
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| NC | µL | µL | µL | µL | µL | µL | µL
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じっけんしゃ
| | DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total
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| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL
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| NC | µL | µL | µL | µL | µL | µL | µL
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Transformation
じっけんしゃ
| Name | Sample | Competent Cells | Total | Plate
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| いでんし | なんちゃらµL | ごにょごにょµL | ほにゃららµL | こーせーぶっしつ
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| いかどうよう | | | |
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| | | | |
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Electrophoresis
じっけんしゃ
| Lane | Sample | Enzyme1 | Enzyme2
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| （°Д°） | | |
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| Lane | Sample | Enzyme1 | Enzyme2
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| （°Д°） | | |
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| Lane | Sample | Enzyme1 | Enzyme2
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| （°Д°） | | |
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"
