Template:Kyoto/Notebook/Sep 9

(Difference between revisions)

Revision as of 17:16, 23 September 2013

Nakagawa

KaiA(plasmid)	10x buffer	dNTPs	MgSO4	Primer(661-RBS-KaiA-fwd)	Primer(kaiA-662-rev-ver2)	Kod-plus ver2	MilliQ	total
1.0	2.5	2.5	1.5	0.75	0.75	0.5	15.5	25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	58.5°C	68°C	--
2min	10s	30s	30s	30cycles

KaiB(plasmid)	10x buffer	dNTPs	MgSO4	Primer(661-RBS-KaiA-fwd-ver2)	Primer(kaiB-663-rev-ver2)	Kod-plus ver2	MilliQ	total
1.0	2.5	2.5	1.5	0.75	0.75	0.5	15.5	25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	58.5°C	68°C	--
2min	10s	30s	30s	30cycles

P-KaiBC(PCR products)	10x buffer	dNTPs	MgSO4	Primer(prefix-P-kaiBC-fwd-ver2)	Primer(P-KaiBC-suffix-rev-verw)	Kod-plus ver2	MilliQ	total
0.7	2.5	2.5	1.5	0.75	0.75	0.5	15.8	25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	58.5°C	68°C	--
2min	10s	30s	30s	30cycles

DT(plasmid)	10x buffer	dNTPs	MgSO4	Primer(664-DT-fwd-ver2)	Primer(DT-suffix-660-rev-ver2)	Kod-plus ver2	MilliQ	total
1.0	2.5	2.5	1.5	0.75	0.75	0.5	15.5	25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	58.5°C	68°C	--
2min	10s	30s	30s	30cycles

@@ Line 15: / Line 15: @@
 |2min||10s||30s||30s||30cycles
 |}
-</div>
-<div class="experiment">
 {| class="wikitable"
 !KaiB(plasmid)||10x buffer||dNTPs||MgSO4||Primer(661-RBS-KaiA-fwd-ver2)||Primer(kaiB-663-rev-ver2)||Kod-plus ver2||MilliQ||total
@@ Line 33: / Line 31: @@
 |-
 |0.7||2.5||2.5||1.5||0.75||0.75||0.5||15.8||25
+|}
+{| class="wikitable"
+!PreDenature||Denature||Annealing||Extension||cycle
+|-
+|94&deg;C||98&deg;C||58.5&deg;C||68&deg;C||--
+|-
+|2min||10s||30s||30s||30cycles
+|}
+{| class="wikitable"
+!DT(plasmid)||10x buffer||dNTPs||MgSO4||Primer(664-DT-fwd-ver2)||Primer(DT-suffix-660-rev-ver2)||Kod-plus ver2||MilliQ||total
+|-
+|1.0||2.5||2.5||1.5||0.75||0.75||0.5||15.5||25
 |}
 {| class="wikitable"