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Template:Kyoto/Notebook/Sep 8
From 2013.igem.org
(Difference between revisions)
(→Gel Extraction) |
(→Gel Extraction) |
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| Line 30: | Line 30: | ||
|1||100bp ladder|| | |1||100bp ladder|| | ||
|- | |- | ||
| - | |3||Pcon-pT181 | + | |3||Pcon-pT181 attenuator||EcoRI & SpeI |
|- | |- | ||
| - | |4||Pcon-pT181 | + | |4||Pcon-pT181 attenuator||EcoRI & SpeI |
|- | |- | ||
|6||Spinach-DT||XbaI & PstI | |6||Spinach-DT||XbaI & PstI | ||
| Line 47: | Line 47: | ||
!Name||concentration[µg/mL]||260/280||260/230 | !Name||concentration[µg/mL]||260/280||260/230 | ||
|- | |- | ||
| - | |Pcon-pT181 | + | |Pcon-pT181 attenuator (EcoRI & SpeI)||5.2||1.82||0.36 |
|- | |- | ||
| - | |Spinach-DT(XbaI & PstI)||8.0||1.87||0.25 | + | |Spinach-DT (XbaI & PstI)||8.0||1.87||0.25 |
|- | |- | ||
| - | |RBS-lacZα-DT(XbaI & PstI)||10.3||1.71||0.54 | + | |RBS-lacZα-DT (XbaI & PstI)||10.3||1.71||0.54 |
|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
| Line 73: | Line 73: | ||
|Ptet-pT181 antisense(EcoRI & PstI)||30.6||1.84||1.16 | |Ptet-pT181 antisense(EcoRI & PstI)||30.6||1.84||1.16 | ||
|- | |- | ||
| - | |pSB4K5(EcoRI & SpeI)||21.8||1.87||0.98 | + | |pSB4K5 (EcoRI & SpeI)||21.8||1.87||0.98 |
|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
| Line 101: | Line 101: | ||
!Name||concentration[µg/mL]||260/280||260/230 | !Name||concentration[µg/mL]||260/280||260/230 | ||
|- | |- | ||
| - | |Pcon-pT181 attenuator(SpeI & PstI)||14.5||1.91||1.09 | + | |Pcon-pT181 attenuator (SpeI & PstI)||14.5||1.91||1.09 |
|- | |- | ||
| - | |Plac(SpeI & PstI)||11.6||1.74||1.19 | + | |Plac (SpeI & PstI)||11.6||1.74||1.19 |
|- | |- | ||
| - | |pSB1C3(EcoRI & SpeI)||5.7||1.74||0.34 | + | |pSB1C3 (EcoRI & SpeI)||5.7||1.74||0.34 |
|- | |- | ||
| - | |pSB1C3(XbaI & PstI)||5.6||2.00||0.37 | + | |pSB1C3 (XbaI & PstI)||5.6||2.00||0.37 |
|} | |} | ||
Revision as of 22:29, 23 September 2013
Contents |
Sep 8
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | |
| 2 | RBS-lysis1-DT | XbaI & PstI |
| 3 | RBS-lysis1-DT | XbaI & PstI |
| 5 | RBS-lysis3-DT | XbaI & PstI |
| 6 | RBS-lysis3-DT | XbaI & PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| RBS-lysis1-DT (XbaI & PstI) | 5.3 | 1.98 | 0.06 |
| RBS-lysis3-DT (XbaI & PstI) | 5.3 | 1.98 | 0.06 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | |
| 3 | Pcon-pT181 attenuator | EcoRI & SpeI |
| 4 | Pcon-pT181 attenuator | EcoRI & SpeI |
| 6 | Spinach-DT | XbaI & PstI |
| 7 | Spinach-DT | XbaI & PstI |
| 9 | RBS-lacZα-DT | XbaI & PstI |
| 10 | RBS-lacZα-DT | XbaI & PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181 attenuator (EcoRI & SpeI) | 5.2 | 1.82 | 0.36 |
| Spinach-DT (XbaI & PstI) | 8.0 | 1.87 | 0.25 |
| RBS-lacZα-DT (XbaI & PstI) | 10.3 | 1.71 | 0.54 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbp ladder | |
| 2 | Ptet-pT181 antisense | EcoRI & PstI |
| 3 | Ptet-pT181 antisense | EcoRI & PstI |
| 5 | pSB4K5 | EcoRI & SpeI |
| 6 | pSB4K5 | EcoRI & SpeI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Ptet-pT181 antisense(EcoRI & PstI) | 30.6 | 1.84 | 1.16 |
| pSB4K5 (EcoRI & SpeI) | 21.8 | 1.87 | 0.98 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbp ladder | |
| 2 | Pcon-pT181 attenuator | SpeI & PstI |
| 3 | Pcon-pT181 attenuator | SpeI & PstI |
| 5 | Plac | SpeI & PstI |
| 6 | Plac | SpeI & PstI |
| 8 | pSB1C3 | EcoRI & SpeI |
| 9 | pSB1C3 | EcoRI & SpeI |
| 11 | pSB1C3 | XbaI & PstI |
| 12 | pSB1C3 | XbaI & PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181 attenuator (SpeI & PstI) | 14.5 | 1.91 | 1.09 |
| Plac (SpeI & PstI) | 11.6 | 1.74 | 1.19 |
| pSB1C3 (EcoRI & SpeI) | 5.7 | 1.74 | 0.34 |
| pSB1C3 (XbaI & PstI) | 5.6 | 2.00 | 0.37 |
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181 anti | 243.0 | 1.90 | 1.81 |
| Pcon-Spinach-DT | 253.7 | 1.57 | 1.51 |
Restriction Enzyme Digestion
| DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| いでんし | なんちゃらµL | ごにょごにょµL | ほにゃららµL | こーせーぶっしつ |
| いかどうよう | ||||
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| （°Д°） |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| （°Д°） |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| （°Д°） |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
PCR
| genome DNA | KOD plus | 10x buffer | dNTP | MgSO4 | SasA_fwd primer | SasA_rev primer | MilliQ | total |
|---|---|---|---|---|---|---|---|---|
| 0.5 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| °C | °C | °C | °C | -- |
| （◎Д◎） |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
