Template:Kyoto/Notebook/Sep 17

From 2013.igem.org

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===PCR===
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<!-- ここから -->
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<div class="experiment">
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<span class="author">No name</span>
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{| class="wikitable"
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!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
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|-
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| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
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|}
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{| class="wikitable"
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!PreDenature||Denature||Annealing||Extension||cycle
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|-
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|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
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|-
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|（◎Д◎）　||　||　||　||
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|}
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</div>
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<!-- ここまでをコピーしてね -->

Revision as of 01:52, 24 September 2013

Sep 17

Liquid Culture

Tatsui and Hirano

Samplemedium
Spi-DTLB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DTLB medium(+Amp)　

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　
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