Template:Kyoto/Notebook/Sep 17
From 2013.igem.org
(Difference between revisions)
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|1||1kbpladder||-- | |1||1kbpladder||-- | ||
|- | |- | ||
- | |2||Pt181 atte -(2)||E+S | + | |2||rowspan="3"|Pt181 atte -(2)||rowspan="3"|E+S |
|- | |- | ||
|3 | |3 | ||
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!Name||concentration[µg/mL]||260/280||260/230 | !Name||concentration[µg/mL]||260/280||260/230 | ||
|- | |- | ||
- | | | + | |PT181-atte E+S||9.2||1.15||0.12 |
- | + | ||
- | + | ||
|} | |} | ||
</div> | </div> | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | {| class="wikitable" | ||
+ | !Lane||DNA||Enzyme | ||
+ | |- | ||
+ | |1||1kbpladder||-- | ||
+ | |- | ||
+ | |2||rowspan="3"|pSB1C3||rowspan="3"|E+S | ||
+ | |- | ||
+ | |3 | ||
+ | |- | ||
+ | |4 | ||
+ | |} | ||
+ | [[File:igku_xxbeforexx.xxx]]<br> | ||
+ | [[File:igku_xxafterxx.xxx]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Name||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |pSB1C3||10.0||1.84||0.40 | ||
+ | |} | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | {| class="wikitable" | ||
+ | !Lane||DNA||Enzyme | ||
+ | |- | ||
+ | |1||1kbpladder||-- | ||
+ | |- | ||
+ | |2||rowspan="3"|pSB4K5||rowspan="3"|X+P | ||
+ | |- | ||
+ | |3 | ||
+ | |- | ||
+ | |4 | ||
+ | |} | ||
+ | [[File:igku_xxbeforexx.xxx]]<br> | ||
+ | [[File:igku_xxafterxx.xxx]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Name||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |pSB4K5||7.6||1.81||0.32 | ||
+ | |} | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | <!-- ここまでをコピーしてね --> | ||
+ | {| class="wikitable" | ||
+ | !Lane||DNA||Enzyme | ||
+ | |- | ||
+ | |1||1kbpladder||-- | ||
+ | |- | ||
+ | |2||rowspan="3"|Pcon-RBS-tetR-DT||rowspan="3"|X+P | ||
+ | |- | ||
+ | |3 | ||
+ | |- | ||
+ | |4 | ||
+ | |} | ||
+ | [[File:igku_xxbeforexx.xxx]]<br> | ||
+ | [[File:igku_xxafterxx.xxx]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Name||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |Pcon-RBS-tetR-DT||8.0||2.40||1.14 | ||
+ | |} | ||
+ | <!-- ここまでをコピーしてね --><!-- ここまでをコピーしてね --> | ||
+ | {| class="wikitable" | ||
+ | !Lane||DNA||Enzyme | ||
+ | |- | ||
+ | |1||1kbpladder||-- | ||
+ | |- | ||
+ | |2||rowspan="3"|Pcon-RBS-tetR-DT||rowspan="3"|X+P | ||
+ | |- | ||
+ | |3 | ||
+ | |- | ||
+ | |4 | ||
+ | |} | ||
+ | [[File:igku_xxbeforexx.xxx]]<br> | ||
+ | [[File:igku_xxafterxx.xxx]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Name||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |Pcon-RBS-tetR-DT||8.0||2.40||1.14 | ||
+ | |} | ||
<!-- ここまでをコピーしてね --> | <!-- ここまでをコピーしてね --> |
Revision as of 02:25, 24 September 2013
Contents |
Sep 17
Liquid Culture
Sample | medium |
---|---|
Spi-DT | LB medium(+CP) |
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp) |
PCR
Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total |
---|---|---|---|---|---|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
96°C | 96°C | 50°C | 60°C | -- |
2m | 10s | 5s | 4m | 30cycles |
Ligation
state | Vector | Inserter | Ligation High ver.2 | ||
---|---|---|---|---|---|
8/28 | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
Gel Extraction
Lane | DNA | Enzyme |
---|---|---|
1 | 1kbpladder | -- |
2 | Pt181 atte -(2) | E+S |
3 | ||
4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
PT181-atte E+S | 9.2 | 1.15 | 0.12 |
Lane | DNA | Enzyme |
---|---|---|
1 | 1kbpladder | -- |
2 | pSB1C3 | E+S |
3 | ||
4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
pSB1C3 | 10.0 | 1.84 | 0.40 |
Lane | DNA | Enzyme |
---|---|---|
1 | 1kbpladder | -- |
2 | pSB4K5 | X+P |
3 | ||
4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
pSB4K5 | 7.6 | 1.81 | 0.32 |
Lane | DNA | Enzyme |
---|---|---|
1 | 1kbpladder | -- |
2 | Pcon-RBS-tetR-DT | X+P |
3 | ||
4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
Pcon-RBS-tetR-DT | 8.0 | 2.40 | 1.14 |
Lane | DNA | Enzyme |
---|---|---|
1 | 1kbpladder | -- |
2 | Pcon-RBS-tetR-DT | X+P |
3 | ||
4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
Pcon-RBS-tetR-DT | 8.0 | 2.40 | 1.14 |