Template:Kyoto/Notebook/Sep 16

From 2013.igem.org

(Difference between revisions)
(→Gel Extraction)
(→Miniprep)
Line 83: Line 83:
!DNA||concentration[µg/mL]||260/280||260/230  
!DNA||concentration[µg/mL]||260/280||260/230  
|-
|-
-
|(・▽・)||　||　||
+
|Dx-lux1||136.7||1.46||1.28
 +
|-
 +
|RBS-laczα-DT||237.6||1.88||1.70
 +
|-
 +
|pSB4K5||148.4||1.55||1.09
 +
|-
 +
|Pcon-KBS-tetR-DT||126.6||1.73||1.36
 +
|-
 +
|9/11Plac1||160.0||1.78||1.28
 +
|-
 +
|9/15Plac2||144.0||1.73||1.81
 +
|-
 +
|9/14Plac332.5||1.51||2.33
 +
|-
 +
|Ptet-RBS-laczα-DT||231.3||1.84||1.57
 +
|-
|}
|}
 +
===PCR===
===PCR===
<!-- ここから -->
<!-- ここから -->

Revision as of 06:16, 24 September 2013

Contents

Sep 16

Liquid Culture

No name

Samplemedium
pSB1C3+P-KaiBC①Plusgrow medium(+CP)
pSB1C3+P-KaiBC②Plusgrow medium(+CP)

Colony PCR

No name

Samplebase pair
pSB1C3+P-KaiBC①283
pSB1C3+P-KaiBC②283
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
2min30s30s18s30cycles

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
11kb ladder
2pT181 attenuatorX+P
3pT181 attenuatorX+P
4pT181 attenuatorX+P
6pSB1C3E+S
7pSB1C3E+S
8pSB1C3E+S
10pSB4K5X+P
11pSB4K5X+P
12pSB4K5X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
pT181 attenuator(X+P)8.21.620.11
pSB1C3(E+S)49.51.480.18
pSB4K5(X+P)18.11.730.57
DT(S+P)70.41.361.02
aptamer12-1R-DT(S+P)42.01.820.47
spinach-DT(S+P)36.81.790.61

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
Dx-lux1136.71.461.28
RBS-laczα-DT237.61.881.70
pSB4K5148.41.551.09
Pcon-KBS-tetR-DT126.61.731.36
9/11Plac1160.01.781.28
9/15Plac2144.01.731.81
9/14Plac332.51.512.33
Ptet-RBS-laczα-DT231.31.841.57

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_16"