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Revision as of 07:10, 24 September 2013
Aromatics Scouts
A Comprehensive Biosensor Toolkit to Profile Aromatics in the Environment
Monitoring aromatics in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.
Transcriptional regulators that each senses a specific class of aromatics were first bioinformatically determined; they were then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and their orthogonality/crosstalk prove that these biosensors are really high-performance to profile aromatics in water.
Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable chemicals into detectable aromatics, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.
We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
Mining aromatics-sensing Biobricks
from the genomic database
Sensor Mining
High-performance, profile-
specific biosensors
Biosensors
Convert the undetectable into
the detectable
Adaptors
Rapidly tell the concentration
of the analyte
Band-pass Filter
Resonate to bridge the gap
between minds
Human Practice
