Template:Kyoto/Notebook/Sep 20

From 2013.igem.org

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(→Ligation)
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!Name||Sample||Competent Cells||Plate
!Name||Sample||Competent Cells||Plate
|-
|-
-
|9/17 IC3-Pcon-pT181attenuator-DT||2µL||20µL||CP
+
|9/17 pSB1C3-Pcon-pT181 attenuator-DT||2 µL||20 µL||CP
|-
|-
-
|9/17 IC3-Pcon-aptamer12-1R-DT||2µL||20µL||CP
+
|9/17 pSB1C3-Pcon-aptamer 12_1R-DT||2 µL||20 µL||CP
|-
|-
-
|9/17 IC3-Pcon-antisense-spinach-DT||2µL||20µL||CP
+
|9/17 pSB1C3-Pcon-antisense-spinach-DT||2 µL||20 µL||CP
|-
|-
-
|9/17 IC3-Pcon-RBS-tetR-DTx2||2µL||20µL||CP
+
|9/17 pSB1C3-Pcon-RBS-tetR-DT x2||2 µL||20 µL||CP
|-
|-
-
|9/14 IC3-PkaiBC||2µL||20µL||CP
+
|9/14 pSB1C3-PkaiBC||2 µL||20 µL||CP
|-
|-
-
|9/14 IC3-RpaB||2µL||20µL||CP
+
|9/14 pSB1C3-RpaB||2 µL||20 µL||CP
|-
|-
-
|9/16 IC3-pT181attenuator(EcoRI&SpeI) ||2µL||20µL||CP
+
|9/16 pSB1C3-pT181 attenuator(EcoRI&SpeI) ||2 µL||20 µL||CP
|-
|-
-
|9/17 IC3-pT181attenuator(XbaI&PstI) ||2µL||20µL||CP
+
|9/17 pSB1C3-pT181 attenuator(XbaI&PstI) ||2 µL||20 µL||CP
|-
|-
-
|9/17IC3-Pcon-spinach-DT||2µL||20µL||CP
+
|9/17 pSB1C3-Pcon-spinach-DT||2 µL||20 µL||CP
|-
|-
-
|9/14 RBS-GFP-DT-PkaiBC||2µL||20µL||CP
+
|9/14 RBS-GFP-DT-PkaiBC||2 µL||20 µL||CP
|-
|-
-
|Plux-RBS-lysis3-DT||2µL||20µL||CP
+
|Plux-RBS-lysis3-DT||2 µL||20 µL||CP
|-
|-
-
|9/20 Ptet-pT181antisense-spinach-DT||2µL||20µL||CP
+
|9/20 Ptet-pT181 antisense-spinach-DT||2 µL||20 µL||CP
|-
|-
-
|9/20 Pcon-spinach-DT(4K5) ||2µL||20µL||K
+
|9/20 Pcon-spinach-DT(pSB4K5) ||2 µL||20 µL||Kan
|-
|-
-
|9/20 Pcon-pT181antisense-spinach-DT(4K5) ||2µL||20µL||K
+
|9/20 Pcon-pT181 antisense-spinach-DT(pSB4K5) ||2 µL||20 µL||Kan
|-
|-
-
|9/20 KaiABC||2µL||20µL||K
+
|9/20 KaiABC||2 µL||20 µL||Kan
|-
|-
-
|9/20 KaiABC||2µL||20µL||K
+
|9/20 KaiABC||2 µL||20 µL||Kan
|-
|-
-
|9/20 Plac-RBS-lysis3-DT||2µL||20µL||Amp
+
|9/20 Plac-RBS-lysis3-DT||2 µL||20 µL||Amp
|-
|-
-
|9/20 Plac-aptamer12-1R||2µL||20µL||Amp
+
|9/20 Plac-aptamer 12_1R||2 µL||20 µL||Amp
|-
|-
-
|9/20 Plux-RBS-GFP-DT-Pcon-RBS-luxR-DT||2µL||20µL||Amp
+
|9/20 Plux-RBS-GFP-DT-Pcon-RBS-luxR-DT||2 µL||20 µL||Amp
|-
|-
-
|Plac-aptamer12-1R-DT||2µL||20µL||Amp
+
|Plac-aptamer12_1R-DT||2 µL||20 µL||Amp
|-
|-
-
|9/20 Plac-aptamer12-1R-DT||2µL||20µL||Amp
+
|9/20 Plac-aptamer12_1R-DT||2 µL||20 µL||Amp
|-
|-
-
|9/20Pcon-pT181attenuator-aptamer-DT||2µL||20µL||Amp
+
|9/20Pcon-pT181attenuator-aptamer-DT||2 µL||20 µL||Amp
|-
|-
-
|9/17 Plac-pT181-attenuatorx2||2µL||20µL||Amp
+
|9/17 Plac-pT181-attenuatorx2||2 µL||20 µL||Amp
|-
|-
-
|9/15 Pcon-RBS-lacZα-DT||2µL||20µL||Amp
+
|9/15 Pcon-RBS-lacZα-DT||2 µL||20 µL||Amp
|-
|-
-
|9/14 Pcon-pT181attenuator-RBS-lacZα-DT||2µL||20µL||Amp
+
|9/14 Pcon-pT181attenuator-RBS-lacZα-DT||2 µL||20 µL||Amp
|-
|-
-
|PUC19||2µL||20µL||Amp
+
|PUC19||2 µL||20 µL||Amp
-
|-
+
-
 
+
|}
|}
</div>
</div>

Revision as of 11:35, 24 September 2013

Contents

Sep 20

Ligation

Okazaki, Kanaoka, Tatsui and Hirano

stateVectorInserterLigation High ver.2
experiment9/18 Plac (SpeI & PstI)3.0 µL9/18 RBS-lysis3-DT (XbaI & PstI)12.4 µL3.5 µL
experiment9/12 Ptet-pT181 antisense1.0 µL9/18 spinach-DT11.9 µL3.5 µL
experiment9/13 Pcon-RBS-luxR-DT2.1 µL9/10 Plux-RBS-GFP-DT5.6 µL3.5 µL
experiment9/13 Pcon-pT181 attenuator (SpeI & PstI)2.0 µL9/18 aptamer 12-1R-DT (XbaI & PstI)6.3 µL3.5 µL
experiment9/18 Plac ②(SpeI & PstI)3.0 µL9/18 aptamer 12-1R-DT(XbaI & PstI)4.5µLx3.5 µL

Transformation

Tatsui

NameSampleCompetent CellsPlate
9/17 pSB1C3-Pcon-pT181 attenuator-DT2 µL20 µLCP
9/17 pSB1C3-Pcon-aptamer 12_1R-DT2 µL20 µLCP
9/17 pSB1C3-Pcon-antisense-spinach-DT2 µL20 µLCP
9/17 pSB1C3-Pcon-RBS-tetR-DT x22 µL20 µLCP
9/14 pSB1C3-PkaiBC2 µL20 µLCP
9/14 pSB1C3-RpaB2 µL20 µLCP
9/16 pSB1C3-pT181 attenuator(EcoRI&SpeI) 2 µL20 µLCP
9/17 pSB1C3-pT181 attenuator(XbaI&PstI) 2 µL20 µLCP
9/17 pSB1C3-Pcon-spinach-DT2 µL20 µLCP
9/14 RBS-GFP-DT-PkaiBC2 µL20 µLCP
Plux-RBS-lysis3-DT2 µL20 µLCP
9/20 Ptet-pT181 antisense-spinach-DT2 µL20 µLCP
9/20 Pcon-spinach-DT(pSB4K5) 2 µL20 µLKan
9/20 Pcon-pT181 antisense-spinach-DT(pSB4K5) 2 µL20 µLKan
9/20 KaiABC2 µL20 µLKan
9/20 KaiABC2 µL20 µLKan
9/20 Plac-RBS-lysis3-DT2 µL20 µLAmp
9/20 Plac-aptamer 12_1R2 µL20 µLAmp
9/20 Plux-RBS-GFP-DT-Pcon-RBS-luxR-DT2 µL20 µLAmp
Plac-aptamer12_1R-DT2 µL20 µLAmp
9/20 Plac-aptamer12_1R-DT2 µL20 µLAmp
9/20Pcon-pT181attenuator-aptamer-DT2 µL20 µLAmp
9/17 Plac-pT181-attenuatorx22 µL20 µLAmp
9/15 Pcon-RBS-lacZα-DT2 µL20 µLAmp
9/14 Pcon-pT181attenuator-RBS-lacZα-DT2 µL20 µLAmp
PUC192 µL20 µLAmp

PCR

Tatsui

Samplebase pair
pT181attenuator(IC3)(XbaI&PstI)--
Pcon-RBS-lacZα-DT--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s3min30cycles

Electrophoresis

No name

LaneSampleEnzyme
1Pcon-RBS-lacZα-DT--
2 1kb ladder--
3IC3-pT181attenuator --
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