Team:Buenos Aires/ res dmrfp

From 2013.igem.org

(Difference between revisions)
(Hibrid promoter induction)
(GFP production in th presence of different arsenite concentrations)
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'''Protocol'''
'''Protocol'''
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Several cultures carrying a plasmid that encodes GFP under an arsenite sensitive promoter (Bba_K1106004) were grown overnight with different arsenite concentration '''(0, 10,..ppb)'''
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Several cultures carrying a plasmid that encodes GFP under an arsenite sensitivnnnnnnnnnnnne promoter (Bba_K1106004) were grown overnight with different arsenite concentration '''(0, 10,..ppb)'''
Fluorescence was measured with a fluorimeter at '''x nm''' exiitation wavelength. All theese data was normalized by the culture density measured by OD.
Fluorescence was measured with a fluorimeter at '''x nm''' exiitation wavelength. All theese data was normalized by the culture density measured by OD.

Revision as of 16:15, 27 September 2013

GFP production in th presence of different arsenite concentrations

Protocol

Several cultures carrying a plasmid that encodes GFP under an arsenite sensitivnnnnnnnnnnnne promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb) Fluorescence was measured with a fluorimeter at x nm exiitation wavelength. All theese data was normalized by the culture density measured by OD.

Results