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Template:Kyoto/Notebook/Sep 15
From 2013.igem.org
(Difference between revisions)
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<div class="experiment"> | <div class="experiment"> | ||
| - | <span class="author"> | + | <span class="author">No Name</span> |
{| class="wikitable" | {| class="wikitable" | ||
! ||DNA||EcorI||SpeI||Buffer||BSA||MilliQ||total | ! ||DNA||EcorI||SpeI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |9/7Pcon-RBS-tetR-DT (EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |9/7Pcon-RBS-tetR-DT (EcoRI & SpeI)||15 µL||1 µL||1 µL||3 µL||3 µL||6 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||0.7 µL||0 µL||0 µL||1 µL||1 µL||7.3 µL||10 µL |
|} | |} | ||
<br> | <br> | ||
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! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI)||6.8 µL||1 µL||1 µL||3 µL||3 µL||15.2 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||0.3 µL||0 µL||0 µL||1 µL||1 µL||7.7 µL||10 µL |
|} | |} | ||
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! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |9/12 Pcon-apt12_1R-DT(EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |9/12 Pcon-apt12_1R-DT(EcoRI & SpeI)||6.6 µL||1 µL||1 µL||3 µL||3 µL||15.4 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||0.3 µL||0 µL||0 µL||1 µL||1 µL||7.7 µL||10 µL |
|} | |} | ||
<br> | <br> | ||
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! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |9/8 Pcon-spin-DT (EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |9/8 Pcon-spin-DT (EcoRI & SpeI)||7.6 µL||1 µL||1 µL||3 µL||3 µL||15.4 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||0.3 µL||0 µL||0 µL||1 µL||1 µL||7.7 µL||10 µL |
|} | |} | ||
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! ||DNA||XbaI||PstI||Buffer||BSA||MilliQ||total | ! ||DNA||XbaI||PstI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI)||7.2 µL||1 µL||1 µL||3 µL||3 µL||14.8 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||0.4 µL||0 µL||0 µL||1 µL||1 µL||7.6 µL||10 µL |
|} | |} | ||
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! ||DNA||XbaI||PstI||Buffer||BSA||MilliQ||total | ! ||DNA||XbaI||PstI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |8/17 RBS-lacZα-DT (XbaI & PstI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |8/17 RBS-lacZα-DT (XbaI & PstI)||5.3 µL||1 µL||1 µL||3 µL||3 µL||16.7 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||0.5 µL||0 µL||0 µL||1 µL||1 µL||7.5 µL||10 µL |
|} | |} | ||
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! ||DNA||EcoRI||XbaI||Buffer||BSA||MilliQ||total | ! ||DNA||EcoRI||XbaI||Buffer||BSA||MilliQ||total | ||
|- | |- | ||
| - | |9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI)||2.9 µL||1 µL||1 µL||3 µL||3 µL||18.5 µL||30 µL |
|- | |- | ||
| - | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | + | |NC||1.5 µL||0 µL||0 µL||1 µL||1 µL||7 µL||10 µL |
|} | |} | ||
<br> | <br> | ||
Revision as of 14:21, 24 September 2013
Contents |
Sep 15
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pλ-luxI | 225 | 1.59 | 0.83 |
Liquid Culture
| Sample | medium |
|---|---|
| 9/13 Pλ-luxI-1 | |
| 9/7 pSB4K5-1 | |
| 9/11 Plac-3 |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | 9/13 Pcon(SpeI & PstI) | 3.6 µL | 9/12 RBS-lacZα-DT(XbaI & PstI) | 4.7 µL | 8 µL |
Restriction Enzyme Digestion
| DNA | EcorI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/7Pcon-RBS-tetR-DT (EcoRI & SpeI) | 15 µL | 1 µL | 1 µL | 3 µL | 3 µL | 6 µL | 30 µL |
| NC | 0.7 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7.3 µL | 10 µL |
| DNA | EcoRI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI) | 6.8 µL | 1 µL | 1 µL | 3 µL | 3 µL | 15.2 µL | 30 µL |
| NC | 0.3 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7.7 µL | 10 µL |
| DNA | EcoRI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/12 Pcon-apt12_1R-DT(EcoRI & SpeI) | 6.6 µL | 1 µL | 1 µL | 3 µL | 3 µL | 15.4 µL | 30 µL |
| NC | 0.3 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7.7 µL | 10 µL |
| DNA | EcoRI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/8 Pcon-spin-DT (EcoRI & SpeI) | 7.6 µL | 1 µL | 1 µL | 3 µL | 3 µL | 15.4 µL | 30 µL |
| NC | 0.3 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7.7 µL | 10 µL |
| DNA | XbaI | PstI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI) | 7.2 µL | 1 µL | 1 µL | 3 µL | 3 µL | 14.8 µL | 30 µL |
| NC | 0.4 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7.6 µL | 10 µL |
| DNA | XbaI | PstI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 8/17 RBS-lacZα-DT (XbaI & PstI) | 5.3 µL | 1 µL | 1 µL | 3 µL | 3 µL | 16.7 µL | 30 µL |
| NC | 0.5 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7.5 µL | 10 µL |
| DNA | EcoRI | XbaI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI) | 2.9 µL | 1 µL | 1 µL | 3 µL | 3 µL | 18.5 µL | 30 µL |
| NC | 1.5 µL | 0 µL | 0 µL | 1 µL | 1 µL | 7 µL | 10 µL |
