Template:Kyoto/Notebook/Aug 16

From 2013.igem.org

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(→Colony PCR)
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|8||BBa_I732020(RBS-lacZα-DT)
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{| class="wikitable"
{| class="wikitable"
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|-
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|2||BBa_I0462(RBS-luxR-DT)(2)
|2||BBa_I0462(RBS-luxR-DT)(2)
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===Transformation===
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<!-- こっから -->
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<div class="experiment">
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<span class="author">じっけんしゃ</span>
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{| class="wikitable"
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!Name||Sample||Competent Cells||Total||Plate
 +
|-
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|pBR322||1&micro;L||100&micro;L(XL-10gold)||101&micro;L||Amp
 +
|-
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|pBR322||1&micro;L||50&micro;L(takara)||51&micro;L||Amp
 +
|-
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|Pbad araC||2&micro;L|||20&micro;L||22&micro;L||K
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|-
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|RBS-luxI-DT||2&micro;L|||20&micro;L||22&micro;L||K
|}
|}

Revision as of 15:38, 24 September 2013

Contents

Aug 16

Colony PCR

A

Samplebase pair
8/15 BBa_I13504(RBS-GFP-DT)(1)1174
8/15 BBa_I13504(RBS-GFP-DT)(2)1174
8/15 BBa_I0462(RBS-luxR-DT)(1)1174
8/15 BBa_I0462(RBS-luxR-DT)(2)1174
8/15 BBa_K748002(lysis3)1053
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/15 BBa_R0061(Plux)344
8/15 BBa_K117000(lysis1)(1)456
8/15 BBa_K117000(lysis1)(2)456
8/15 BBa_B0015(DT)443
8/15 BBa_I732020(RBS-lacZα-DT)703
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s40s30cycles

Master Plate

A

NumberUse LB plate (+CP)
1BBa_I13504(RBS-GFP-DT)(1)
2BBa_I13504(RBS-GFP-DT)(2)
3BBa_K748002(lysis3)
4BBa_R0061(Plux)
5BBa_K117000(lysis1)(1)
6BBa_K117000(lysis1)(2)
7BBa_B0015(DT)
8BBa_I732020(RBS-lacZα-DT)
NumberUse LB plate (+Amp)
1BBa_I0462(RBS-luxR-DT)(1)
2BBa_I0462(RBS-luxR-DT)(2)

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
pBR3221µL100µL(XL-10gold)101µLAmp
pBR3221µL50µL(takara)51µLAmp
Pbad araC2µL20µL22µLK
RBS-luxI-DT2µL20µL22µLK

Liquid Culture

A

Samplemedium
8/15 BBa_I13504(RBS-GFP-DT))(1)Plusgrow medium (+CP)
8/15 BBa_I13504(RBS-GFP-DT)(2)Plusgrow medium (+CP)
8/15 BBa_I0462(RBS-luxR-DT)(2)Plusgrow medium (+Amp)
8/15 BBa_K748002(lysis3)Plusgrow medium (+CP)
8/15 BBa_R0061(Plux)Plusgrow medium (+CP)
8/15 BBa_K117000(lysis1)(1)Plusgrow medium (+CP)
8/15 BBa_K117000(lysis1)(2)Plusgrow medium (+CP)
8/15 BBa_B0015(DT)Plusgrow medium (+CP)
8/15 BBa_I732020(RBS-lacZα-DT)Plusgrow medium (+CP)
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