"
Team:Newcastle
From 2013.igem.org
| Line 90: | Line 90: | ||
<hr/> | <hr/> | ||
<p> | <p> | ||
| - | Our project focuses on the creation and applications of L-forms: bacteria that grow without a cell wall. We propose | + | Our project focuses on the creation and applications of L-forms: bacteria that grow without a cell wall. We propose L-forms as a novel chassis for synthetic biology. Our principle BioBrick switches <i>Bacillus subtilis</i> cells between rod-shape and L-form. |
</p> | </p> | ||
<p> | <p> | ||
Revision as of 11:50, 26 September 2013
Our Project
Our project focuses on the creation and applications of L-forms: bacteria that grow without a cell wall. We propose L-forms as a novel chassis for synthetic biology. Our principle BioBrick switches Bacillus subtilis cells between rod-shape and L-form.
We will use microfluidics to attempt genome shuffling and shape-shifting. It is easier to fuse bacteria without cell walls. Fusion will cause genetic recombination, allowing directed evolution. We will put L-forms in moulds to observe if they adopt different shapes.
L-forms exist symbiotically in plants, which we will visualise by growing GFP labelled L-forms inside seedlings. L-forms could be engineered to supply nutrients to their host. L-forms are osmotically sensitive, giving biosecurity that they lyse if they escape from the plant.
As outreach we reflected upon our project's implications with stakeholders, created a BioGame for the public and developed a workshop for those new to modelling. Finally, we evaluated the relationship between synthetic biology and architecture.
