Template:Kyoto/Notebook/Aug 22

From 2013.igem.org

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</div>
</div>
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===Miniprep===
+
===Electrophoresis===
<div class="experiment">
<div class="experiment">
-
<span class="author">Ashida</span>
+
<span class="author">じっけんしゃ</span>
-
{|class="wikitable"  
+
{| class="wikitable"
-
!DNA||concentration[&micro;g/mL]||260/280||260/230
+
!Lane||Sample
|-
|-
-
|8/22 RBS-lysis (1)||128||1.77||2.29
+
|1||100bp ladder
|-
|-
-
|8/22 RBS-lysis (2)||96||1.80||2.07
+
|2||RBS-lysis1 -(1)
|-
|-
-
|8/22 pSB1C3 (1)||108||1.55||1.45
+
|3||RBS-lysis1 -(2)
|-
|-
-
|8/22 pSB1C3 (2)||130||1.69||1.76
+
|4||RBS-lysis2 -(1)
 +
|-
 +
|5||RBS-lysis2 -(2)
 +
|-
 +
|6||RBS(NC) -(1)
 +
|-
 +
|7||RBS(NC) -(1)
 +
|-
 +
|8||NC
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||1kb ladder
 +
|-
 +
|2||Ptet-RBS-tetR-DT -(1)
 +
|-
 +
|3||Ptet-RBS-tetR-DT -(2)
 +
|-
 +
|4||Ptet(NC) -(1)
 +
|-
 +
|5||Ptet(NC) -(2)
 +
|-
 +
|6||Plux-RBS-GFP-DT -(1)
 +
|-
 +
|7||pSB1C3 -(1)
 +
|-
 +
|8||pSB1C3 -(2)
 +
|-
 +
|9||NC
 +
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||1kb ladder
 +
|-
 +
|2||Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT -(1)
 +
|-
 +
|3||Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT -(2)
 +
|-
 +
|4||Pcon-RBS-luxR -(1)
 +
|-
 +
|5||Pcon-RBS-luxR -(2)
 +
|-
 +
|6||NC
 +
|-
 +
|7||1kb ladder
|}
|}
 +
[[File:igku_xxxxxx.xxx]]<br>
</div>
</div>

Revision as of 02:49, 25 September 2013

Contents

Aug 22

Liquid culture

Nakamoto

SampleMedium
8/21 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT (1)8/21 Plusgrow medium (+Amp)
8/21 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT (2)8/21 Plusgrow medium (+Amp)
8/21 Pcon-RBS-GFP-DT control (1)8/21 Plusgrow medium (+Amp)
8/21 Pcon-RBS-GFP-DT control (2)8/21 Plusgrow medium (+Amp)
8/21 RBS-lysis1 (1)8/21 Plusgrow medium (+Amp)
8/21 RBS-lysis1 (2)8/21 Plusgrow medium (+Amp)
8/21 RBS control (1)8/21 Plusgrow medium (+Amp)
8/21 RBS control (2)8/21 Plusgrow medium (+Amp)
8/21 Ptet(pm)-RBS-tetR-DT (1)8/21 Plusgrow medium (+Amp)
8/21 Ptet(pm)-RBS-tetR-DT (2)8/21 Plusgrow medium (+Amp)
8/21 Ptet(pm) control (1)8/21 Plusgrow medium (+Amp)
8/21 Ptet(pm) control (2)8/21 Plusgrow medium (+Amp)
8/21 Plux-RBS-GFP-DT (1)8/21 Plusgrow medium (+Amp)
8/21 pSB1C3(BBa_J04450) (1)8/21 Plusgrow medium (+Amp)
8/21 pSB1C3(BBa_J04450) (2)8/21 Plusgrow medium (+Amp)

incubate 37°C 10hour

Colony PCR

Kojima

Samplebase pair
8/21 RBS-lysis1(1)400
8/21 RBS-lysis1(2)400
8/21 RBS control(1)--
8/21 RBS control(2)--
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles
Samplebase pair
8/21 Pcon-GFP-DT-Pcon-RBS-luxR-DT (1)2143
8/21 Pcon-GFP-DT-Pcon-RBS-luxR-DT (2)2143
8/21 Pcon-RBS-luxR control(1)--
8/21 Pcon-RBS-luxR control(2)--
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s2min30cycles
Samplebase pair
8/21 Ptet-RBS-tetR-DT (1)1216
8/21 Ptet-RBS-tetR-DT (2)1216
8/21 Ptet control (1)--
8/21 Ptet control (2)--
8/21 Plux-RBS-GFP-DT (1)1227
8/21 pSB1C3(BBa_J04450) (1)1353
8/21 pSB1C3(BBa_J04450) (2)1353
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles

Electrophoresis

じっけんしゃ

LaneSample
1100bp ladder
2RBS-lysis1 -(1)
3RBS-lysis1 -(2)
4RBS-lysis2 -(1)
5RBS-lysis2 -(2)
6RBS(NC) -(1)
7RBS(NC) -(1)
8NC

File:Igku xxxxxx.xxx

LaneSample
11kb ladder
2Ptet-RBS-tetR-DT -(1)
3Ptet-RBS-tetR-DT -(2)
4Ptet(NC) -(1)
5Ptet(NC) -(2)
6Plux-RBS-GFP-DT -(1)
7pSB1C3 -(1)
8pSB1C3 -(2)
9NC

File:Igku xxxxxx.xxx

LaneSample
11kb ladder
2Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT -(1)
3Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT -(2)
4Pcon-RBS-luxR -(1)
5Pcon-RBS-luxR -(2)
6NC
71kb ladder

File:Igku xxxxxx.xxx

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp----
28/22 pT181 attenuator (1)EcoRISpeI
38/22 pT181 attenuator (1)EcoRI--
48/22 pT181 attenuator (1)--SpeI
58/22 pT181 attenuator (1)----
68/22 pT181 attenuator (1)XbaIPstI
78/22 pT181 attenuator (1)XbaI--
88/22 pT181 attenuator (1)--PstI
98/22 pT181 attenuator (1)----
108/22 Fusion6 antisense (1)EcoRISpeI
118/22 Fusion6 antisense (1)EcoRI--
128/22 Fusion6 antisense (1)--SpeI
138/22 Fusion6 antisense (1)----
14100bp----

Igku Aug22electrophoresis.jpg

LaneSampleEnzyme1Enzyme2
1100bp----
28/22 Fusion6 antisense (1)XbaIPstI
38/22 Fusion6 antisense (1)XbaI--
48/22 Fusion6 antisense (1)--PstI
58/22 Fusion6 antisense (1)----
68/22 Fusion1 antisense (1)EcoRISpeI
78/22 Fusion1 antisense (1)EcoRI--
88/22 Fusion1 antisense (1)--SpeI
98/22 Fusion1 antisense (1)----
108/22 Fusion1 antisense (1)XbaIPstI
118/22 Fusion1 antisense (1)XbaI--
128/22 Fusion1 antisense (1)--PstI
138/22 Fusion1 antisense (1)----
14100bp----

Igku Aug22electrophoresis2.jpg

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