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Team:ZJU-China/Notebook/LabNotes/June
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| - | June 1st||Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P. | + | |June 1st||Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P. |
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| - | June 2nd||Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP). | + | |June 2nd||Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP). |
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| - | June 3rd||Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time. | + | |June 3rd||Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time. |
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| - | June 4th||Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st. | + | |June 4th||Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st. |
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| - | June 5th||Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010. | + | |June 5th||Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010. |
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| - | June 6th||Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes. | + | |June 6th||Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes. |
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| - | June 7th||Plasmid purification of 23L part. Store strains in -80°C. | + | |June 7th||Plasmid purification of 23L part. Store strains in -80°C. |
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| - | June 8th||Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP. | + | |June 8th||Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP. |
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| - | June 9th||Small-scale plasmid purification of GFP. | + | |June 9th||Small-scale plasmid purification of GFP. |
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| - | June 10th|| | + | |June 10th|| |
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| - | June 11th||Preliminary experiment using new competent cell of DH52, BL21, and ??????? | + | |June 11th||Preliminary experiment using new competent cell of DH52, BL21, and ??????? |
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| - | June 12th||Pick up colonies of GFP and R0010 and cultivate in LB+A medium. | + | |June 12th||Pick up colonies of GFP and R0010 and cultivate in LB+A medium. |
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| - | June 13th-30th||Final examinations for the semester. | + | |June 13th-30th||Final examinations for the semester. |
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Revision as of 15:49, 25 September 2013
Lab Notes: June
| June 1st | Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P. |
| June 2nd | Gel electrophoresis to analyze restriction fragments. Transformations of several parts (BBa_K6008002, BBa_K411003, 23L, 17F, GFP). |
| June 3rd | Pick up three colonies of part 17F and cultivate in LB+A medium. Transformations of 23L and 5-1D R0010 for another time. |
| June 4th | Pick up colonies of 23L terminator --- successful! Transformations of 17F, GFP, and plasmids mentioned on June 1st. |
| June 5th | Find monoclone of BBa_K6008002 (cultured in June 2nd). Transformation of part DH10. Pick up colonies of 5-1D R0010. |
| June 6th | Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes. |
| June 7th | Plasmid purification of 23L part. Store strains in -80°C. |
| June 8th | Transformations of parts R0010, GFP and BBa_K411003. Pick up colonies of R0010 and GFP. |
| June 9th | Small-scale plasmid purification of GFP. |
| June 10th | |
| June 11th | Preliminary experiment using new competent cell of DH52, BL21, and ??????? |
| June 12th | Pick up colonies of GFP and R0010 and cultivate in LB+A medium. |
| June 13th-30th | Final examinations for the semester. |
