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Team:NU Kazakhstan/Aptamers
From 2013.igem.org
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| + | <h3><center>Selection of ssDNA Aptamers for Use in Detection of Cancer Biomarker-Carcinoembryonic Antigen</center></h3> | ||
| + | <div>1. <b>PCR AMPLIFICATION CYCLE AND PRIMER CONCENTRATION OPTIMIZATION EXPERIMENTS</b></div> | ||
| + | <div><u>PCR optimization conditions: our reagent and Qiagen reagents</u></div> | ||
| + | <p>Since PCR products, that were obtained using combination of our own reagents (Taq.pol from New England Biolabs, MgCl2 from NCB and so on), were faint on gel I decided to use Qiagen PCR amplification kit, which I found in the freezer. I run two reactions at the same. As the result bands for PCR products from Qiagen were more visible and stronger.</p> | ||
| + | <br/><img src="https://static.igem.org/mediawiki/2013/4/4b/Selex_1.png" alt="" /> | ||
| + | <p>Conclusion: use Qiagen reagent kit for further PCR amplifications</p> | ||
| + | <p>Problem: still having multiple bands and smears</p> | ||
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Revision as of 18:04, 25 September 2013
