Team:Washington/iGEMVectors

From 2013.igem.org

(Difference between revisions)

Revision as of 01:47, 26 September 2013

iGEM Vector Information

Vector	Copy Number	Equivalent	Antibiotic	Length	Genbank / ApE files
pSB1C3	100-300	ColE1,pMB1 (pUC19)	Chloramphenicol	2070 bp	pSB1C3(std).gb
pSB1A3	100-300	ColE1,pMB1 (pUC19)	Ampicillin	2155 bp	pSB1A3(std).gb
pSB3K3	20-30	p15A (pMR101)	Kanamycin	2750 bp	pSB3K3(std).gb
pSB4A5	~5	RepA (pSC101)	Ampicillin	2037 bp	pSB4A5(std).gb
pSB3T5	20-30	p15A (pMR101)	Tetracycline	3252 bp	pSB3T5(std).gb

Vector files are in Genbank compatible ApE format saved as .gb files.
The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.

Vector Equivalency Chart

Source Vector	Origin	Copy Number	Antibiotic	Biobrick Equivalent	Antibiotic	Biobrick Name
pJT119b	ColE1,pMB1	100-300	Chloramphenicol	pSB1C3	Chloramphenicol	pBB_GonG
pPLPCB	p15A	20-30	Spectinomycin	pSB3T5	Tetracycline	pBB_PCB
pEO100c	pSC101	~5	Ampicillin	pSB4A5	Ampicillin	pBB_RoffG
pJT106b	pSC101	~5	Ampicillin	pSB4A5	Ampicillin	pBB_RonL

pCph8.gb

@@ Line 83: / Line 83: @@
    <th>Biobrick Equivalent</th>
    <th>Antibiotic</th>
+  <th>Biobrick Name</th>
 </tr>
 <tr>
@@ Line 91: / Line 92: @@
    <td>pSB1C3</td>
    <td>Chloramphenicol</td>
+  <th>pBB_GonG</th>
 </tr>
 <tr>
@@ Line 99: / Line 101: @@
    <td>pSB3T5</td>
    <td>Tetracycline</td>
+  <th>pBB_PCB</th>
 </tr>
 <tr>
@@ Line 107: / Line 110: @@
    <td>pSB4A5</td>
    <td>Ampicillin</td>
+  <th>pBB_RoffG</th>
 </tr>
 <tr>
@@ Line 115: / Line 119: @@
    <td>pSB4A5</td>
    <td>Ampicillin</td>
+  <th>pBB_RonL</th>
 </tr>
 </table>