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| - | <p style="padding-top: 0pt; " class="Free_Form">Abstract<br /></p>
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| - | <p class="Free_Form"> Human Practice project to provide an understanding of the biology sintestis which has profound implications for the whole society. Impact is felt by the peoples have in the fields of food, energy and health. We want to know the response of the synthetic biology community and their hopes for the future with this synthetic biology. Discussing about synthetic biology, a very important influence on human life, especially in the field of industry.<br /></p>
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| - | <p class="Free_Form"> As a young generation who aspire to a glorious future, we believe that synthetic biology is capable of providing extensive employment opportunities for the young generation. We also interact with the public and talk about the impact of synthetic biology, and they give positive feedback about it. We conducted a survey directly to the society, including surveys to high school students and also to the government agencies. And they provide a different view than ever of synthetic biology. Now they believe that synthetic biology is not just a science, but a science that utilizes all elements of life for the common good.<br /></p>
| + | E. coli which able to measure the level of sugar in honey by emitting light |
| - | <p class="Free_Form">We have conducted a survey with related topic about “Synthetic Biology” in Sumbawa island especially Sumbawa Regency and West Sumbawa Regency. Total our participant there are 235 person. We divided our respondent to six (6) group; students, teachers, seller, farmer, employer and unemployed.<br /></p>
| + | Glucose and fructose are major sugar component of honey. Sumbawa honey is famous as central production of forest honey in Indonesia. Sumbawa honey is also protected as geographical indication by Indonesian patent office. Taneoka et al (2009) has created a fusion of mutant glucose binding protein and firefly luciferase, which able to measure glucose level by emitting light - intended initially for blood glucose sensor application. In this project, we plan to create this construct in Biobrick format, and evaluate the ability of transgenic E. coli for the measurement of glucose in Sumbawa honey. Our final goal is to create a device which can be used for quality control of Sumbawa honey. The basic method is rapid detection for glucose and fructose by construct BioBrick which is fussion between Glucose Binding Protein (GBP) with Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP). The BioBrick construction named "ECONEY”. |
| - | <p class="Free_Form"><br /></p>
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| - | <p class="Free_Form">Result and Discussion<br /></p>
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| - | <p class="Free_Form">1. Have you ever heard about “Synthetic Biology”?<br /></p>
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| - | <p class="Free_Form">The diagram shows the survey result wether society have ever heard about synthetic biology or not.<br /></p>
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| - | <div class="paragraph Free_Form"><div style="height: 243px; width: 574px; height: 243px; position: relative; width: 574px; " class="tinyText style_SkipStroke_1 inline-block"><img src="https://static.igem.org/mediawiki/2013/5/5c/DroppedImage_1.jpg" alt="" style="border: none; height: 243px; width: 574px; " /></div><br /></div>
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| - | <p class="Free_Form"> As the pie chart below showed, most of student have heard about synthetic biology in school from texbook of Biologi when they attand at 3rd grade in high school. The publict have heard about synthetic biologi from mass media, such as newspaper, magazine, televition, etc.<br /></p>
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| - | <p class="Free_Form"><br /></p>
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| - | <ol>
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| - | <li style="line-height: 23px; padding-left: 19px; text-indent: -19px; " class="full-width" value="2">
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| - | <p style="text-indent: -19px; " class="paragraph_style_1"><span style="font-size: 15px; " class="箇条書き項目">2.</span><span style="width: 6px; " class="inline-block"></span>Views on synthetic biology<br /></p>
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| - | </li>
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| - | </ol>
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| - | <div class="paragraph paragraph_style_2"><div style="height: 240px; width: 620px; height: 240px; position: relative; width: 620px; " class="tinyText style_SkipStroke_1 inline-block"><img src="https://static.igem.org/mediawiki/2013/b/b2/DroppedImage_1.png" alt="" style="border: none; height: 240px; width: 620px; " /></div><br /></div>
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| - | <p class="paragraph_style_2"> The pie chart showed that “Views of the public about synthetic biology” before and after we do presentation about synthetic biology.<br /></p>
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| - | <p class="paragraph_style_2"> People who answered that synthetic biology is useful increased by 4% after presentation and people who answered useless decreased by 4%.<br /></p>
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| - | <p class="paragraph_style_2"> It shows that synthetic biology has been received by society and they gave positive feedback about it. <br /></p>
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| - | <p class="paragraph_style_2"><br /></p>
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| - | <ol>
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| - | <li style="line-height: 23px; padding-left: 19px; text-indent: -19px; " class="full-width" value="3">
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| - | <p style="text-indent: -19px; " class="paragraph_style_3"><span style="font-size: 15px; " class="箇条書き項目">3.</span><span style="width: 6px; " class="inline-block"></span>Understanding of synthetic biology<br /></p>
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| - | </li>
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| - | </ol>
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| - | <div class="paragraph paragraph_style_4"><div style="height: 251px; width: 620px; height: 251px; position: relative; width: 620px; " class="tinyText style_SkipStroke_1 inline-block"><img src="https://static.igem.org/mediawiki/2013/4/4f/DroppedImage_2.png" alt="" style="border: none; height: 252px; width: 620px; " /></div><br /></div>
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| - | <p class="paragraph_style_4"> As the previous diagram, we also asked about society understanding about synthetic biology. It shows that (As in the previous pie chart, we also asked about the public's understanding of synthetic biology. Diagram shows that) Before we make a presentation using synthetic biology posters, public understanding as follows:<br /></p>
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| - | <p class="paragraph_style_4">87 people (37%) with a good understanding of synthetic biology<br /></p>
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| - | <p class="paragraph_style_4">98 people (42%) of synthetic biology to understand well enough<br /></p>
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| - | <p class="paragraph_style_4">50 people (21%) do not understand synthetic biology<br /></p>
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| - | <p class="paragraph_style_4"><br /></p>
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| - | <p class="paragraph_style_4">After we did the presentation, understanding community being as follows:<br /></p>
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| - | <p class="paragraph_style_4">125 people (53%) with a good understanding of synthetic biology or an increase of 16%<br /></p>
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| - | <p class="paragraph_style_4">94 people (40%) of synthetic biology to understand well enough or decreased by 2%<br /></p>
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| - | <p class="paragraph_style_4">16 people (7%) did not understand synthetic biology or decreased by 14%<br /></p>
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| - | <p class="paragraph_style_4"><br /></p>
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| - | <p class="paragraph_style_4">It shows that most of society have understood well enough about synthetic.<br /></p>
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| - | <p class="paragraph_style_4"><br /></p>
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| - | <ol>
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| - | <li style="line-height: 23px; padding-left: 19px; text-indent: -19px; " class="full-width" value="4">
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| - | <p style="text-indent: -19px; " class="paragraph_style_5"><span style="font-size: 15px; " class="箇条書き項目">4.</span><span style="width: 6px; " class="inline-block"></span>Product of synthetic biology That mentioned By respondent <br /></p>
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| - | <div class="paragraph paragraph_style_6"><div style="height: 301px; width: 620px; height: 301px; position: relative; width: 620px; " class="tinyText style_SkipStroke_1 inline-block"><img src="https://static.igem.org/mediawiki/2013/0/00/DroppedImage.jpg" alt="" style="border: none; height: 301px; width: 620px; " /></div><br /></div>
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| - | <p class="paragraph_style_6"> The diagram shows that the public is able to mention the products of synthetic biology, although they do not fully understand the process and the materials used to manufacture these products.<br /></p>
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| - | <p class="paragraph_style_6"><br /></p>
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| - | <ol>
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| - | <li style="line-height: 23px; padding-left: 19px; text-indent: -19px; " class="full-width" value="5">
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| - | <p style="text-indent: -19px; " class="paragraph_style_7"><span style="font-size: 15px; " class="箇条書き項目">5.</span><span style="width: 6px; " class="inline-block"></span>Curiosity(wishing) of Synthetic Biology<br /></p>
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| - | </li>
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| - | </ol>
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| - | <div class="paragraph paragraph_style_8"><div style="height: 288px; width: 620px; height: 288px; position: relative; width: 620px; " class="tinyText style_SkipStroke_1 inline-block"><img src="https://static.igem.org/mediawiki/2013/5/54/DroppedImage.png" alt="" style="border: none; height: 289px; width: 620px; " /></div><br /></div>
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| - | <p class="paragraph_style_8"> At the end of the survey, we asked the public's willingness to synthetic biology, the answer as follows:<br /></p>
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| - | <p class="paragraph_style_8">102 people (43%) want to learn more in synthetic biology, and<br /></p>
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| - | <p class="paragraph_style_8">133 people (57%) would like to use or consume products of synthetic biology.<br /></p>
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