"
Team:Groningen/20 June 2013
From 2013.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Added 1 ul Serva/100 ml 1.5% agarose gel. | Added 1 ul Serva/100 ml 1.5% agarose gel. | ||
<br/>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water. | <br/>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water. | ||
| - | </ | + | <br/>Made a 2x dilution of the loading buffer. |
The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made: | The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made: | ||
| - | 7 ul MQ water | + | <br/>7 ul MQ water |
| - | 1 ul 10x buffer | + | <br/>1 ul 10x buffer |
| - | 1 ul restriction enzyme | + | <br/>1 ul restriction enzyme |
| - | 2 ul DNA solution | + | <br/>2 ul DNA solution |
The incubation is done at 37 degrees Celsius for 2 hours. | The incubation is done at 37 degrees Celsius for 2 hours. | ||
After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel. | After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel. | ||
| - | The gel electrophorese is done for 25 minutes at 90V. | + | <br/>The gel electrophorese is done for 25 minutes at 90V. |
The picture is coming soon. | The picture is coming soon. | ||
Revision as of 14:34, 21 June 2013
Added 1 ul Serva/100 ml 1.5% agarose gel.
Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water.
Made a 2x dilution of the loading buffer.
The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made:
7 ul MQ water
1 ul 10x buffer
1 ul restriction enzyme
2 ul DNA solution
The incubation is done at 37 degrees Celsius for 2 hours.
After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel.
The gel electrophorese is done for 25 minutes at 90V.
The picture is coming soon.
