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Team:NJU China/Project/Brain
From 2013.igem.org
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| - | + | <a>Liver:</a> | |
| + | <span>For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study[1], we knew that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells. We tried to engineer the pre-S1 from HBV envelope protein to the lamp 2b.</BR> | ||
| + | Therefore we cloned the pre-S1 into lamp 2b, and we choose pcDNA 3.1(+) as our backbone.</span> | ||
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| + | </h3> | ||
</div> | </div> | ||
<div class="ss-right"> | <div class="ss-right"> | ||
<h3> | <h3> | ||
| - | <a> | + | <a>Results for liver targeting:</a> |
| - | <span> | + | <span>To produce the exosomes that have pre-S1 on their surface for liver targeting, we first transfected the exosome-producing cells, HEK 293T cells, with the plasmid encoding the fusion protein of lamp 2b and pre-S1 peptide.</span> |
| - | + | ||
</h3> | </h3> | ||
Revision as of 12:28, 26 September 2013
RESULTS
Brain
2013
Liver:
For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study[1], we knew that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells. We tried to engineer the pre-S1 from HBV envelope protein to the lamp 2b.
Therefore we cloned the pre-S1 into lamp 2b, and we choose pcDNA 3.1(+) as our backbone.
