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Team:Paris Saclay/Labwork
From 2013.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
07/03/13 | 07/03/13 | ||
| - | * Colony PCR of Bba_K1155000 | + | * Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3 |
| + | * New culture of Bba_K1155000 | ||
| + | |||
| + | 07/04/13 | ||
| + | * Estimation of Pfnr Colony PCR size fragments thanks to Gene manager | ||
| + | * Electrophoresis of Colony PCR products [[File:Psright.png|20px]] | ||
| + | * Stock of Bba_K1155000 | ||
| + | * Culture of Bba_K1155000 | ||
| + | * Extraction of Bba_K1155000 from DH5α | ||
| + | * Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again | ||
| + | |||
| + | 07/05/13 | ||
| + | * Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI [[File:Pswrong.png|20px]] | ||
=='''Bba_K1155001'''== | =='''Bba_K1155001'''== | ||
| Line 32: | Line 44: | ||
07/02/13 | 07/02/13 | ||
| - | * Tranformation of Bba_K592009 in DH5α | + | * Tranformation of Bba_K592009 in DH5α [[File:Psright.png|20px]] |
| + | |||
| + | 07/03/13 | ||
| + | * New culture of Bba_K592009 | ||
=='''Bba_K1155004'''== | =='''Bba_K1155004'''== | ||
| Line 43: | Line 58: | ||
07/02/13 | 07/02/13 | ||
| - | * Tranformation of Bba_I732017 in DH5α | + | * Tranformation of Bba_I732017 in DH5α [[File:Psright.png|20px]] |
| + | |||
| + | 07/03/13 | ||
| + | * New culture of Bba_I732017 | ||
=='''Bba_K1155008'''== | =='''Bba_K1155008'''== | ||
Revision as of 14:17, 26 September 2013
Contents |
Labwork overview
Bba_K1155000
07/01/13
- Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
- Ligation of PSB1C3 and Pfnr
07/02/13
- Tranformation of Bba_K1155000 in DH5α
07/03/13
- Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
- New culture of Bba_K1155000
07/04/13
- Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
- Electrophoresis of Colony PCR products
- Stock of Bba_K1155000
- Culture of Bba_K1155000
- Extraction of Bba_K1155000 from DH5α
- Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again
07/05/13
- Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
Bba_K1155001
Bba_K1155002
Bba_K1155003
07/02/13
- Tranformation of Bba_K592009 in DH5α
07/03/13
- New culture of Bba_K592009
Bba_K1155004
Bba_K1155005
Bba_K1155006
Bba_K1155007
07/02/13
- Tranformation of Bba_I732017 in DH5α
07/03/13
- New culture of Bba_I732017
Bba_K1155008
Bba_K1155009
07/01/13
- Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans
